Search for dissertations about: "refolding"
Showing result 11 - 15 of 27 swedish dissertations containing the word refolding.
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11. Engineering of the Ultra-stable Cystine Knot Framework of Microproteins : Design, Chemical Synthesis and Structural Studies
Abstract : Ultra-stable cystine knotted microproteins, in which two disulfides and their connecting backbones form a circle that is penetrated by the third disulfide bonds, have attracted high interest due to their resistance to degradation in vitro and potential for the development of peptide drugs. This thesis gives new insights into engineering of that framework of microproteins, including approaches to their chemical synthesis, backbone engineering, structural and biological evaluations. READ MORE
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12. On the mechanism of Urea-induced protein denaturation
Abstract : It is well known that folded proteins in water are destabilized by the addition of urea. When a protein loses its ability to perform its biological activity due to a change in its structure, it is said to denaturate. READ MORE
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13. Structural characterization of Deinococcus radiodurans phytochrome in solution
Abstract : Phytochromes are red/far-red photoreceptors which control a wide spectrum of biological processes inplants, fungi, and bacteria. Although phytochromes have been extensively studied, their mechanism ofaction still remains elusive. The signaling mechanism suggested by crystal structures involves refoldingof the so-called PHY tongue. READ MORE
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14. Lipoprotein lipase-unstable on purpose?
Abstract : Lipoprotein lipase (LPL) is a central enzyme in lipid metabolism. It is a non-covalent, homodimeric and N-glycosylated protein, which is regulated in a tissue-specific manner and is dependent on an activator protein, apolipoprotein CII. Dissociation of active LPL dimers to monomers leads to loss of activity. READ MORE
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15. Molecular probes as protein analysis tools in biotechnology
Abstract : Protein-labeling today is a work of art, in vivo studies of proteins or other molecules can easily be performed, and the movement of the labeled molecule can be followed in real time. Labeling in vitro gives enormous amount of data in labs all over the world on a daily basis, where protein-protein, protein-DNA or other interactions are studied. READ MORE