Search for dissertations about: "reverse genetics"

Showing result 1 - 5 of 34 swedish dissertations containing the words reverse genetics.

  1. 1. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Ann-Sofie Rytting; Uppsala universitet.; [1998]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. READ MORE

  2. 2. Repetitive DNA in search of a function - a study of telomeric and centromeric sequences in Chironomus

    University dissertation from Department of Genetics, Lund University

    Author : Casimiro Castillejo-Lopez; Lund University.; Lunds universitet.; [1998]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chironomus; CENP-B box; mobile elements; reverse transcriptase; gene conversion; satellite; repetitive DNA; Telomere; centromere; Genetics; cytogenetics; Genetik; cytogenetik;

    Abstract : Repetitive DNA is quantitatively the main component of telomeres and centromeres, structures responsible for maintenance of the eukaryotic chromosome. The telomere is the specialized nucleoprotein complex that terminates linear chromosomes. READ MORE

  3. 3. Genetic Characterization of Bone and Soft Tissue Tumors

    University dissertation from Department of Clinical Genetics, Lund University

    Author : Anna Dahlen; Lund University.; Lunds universitet.; [2005]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Clinical genetics; Klinisk genetik; cytogenetik; Genetik; cytogenetics; mesenchymal tumor; pericytoma; Genetics; chondroma; lipoma;

    Abstract : Bone and soft tissue tumors (BSTT) constitute a heterogeneous group of neoplasms of mesenchymal and neuroectodermal origin. Although many BSTT are rare, it has become clear that BSTT are characterized by recurrent acquired chromosomal aberrations, and the general aim of this thesis have been to apply molecular genetic and molecular cytogenetic techniques to further characterize recurrent breakpoints and deletions, and to search for candidate target genes. READ MORE

  4. 4. Telomeric DNA in Chironomus, a naturally telomerase-free system

    University dissertation from Department of Genetics, Lund University

    Author : Monika Rosén; Lund University.; Lunds universitet.; [2001]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biology; Chironomus; extrachromosomal telomeric repeats; RNA-DNA complex; 3´overhang; telocentric chromosome end; Telomere; complex repeats; Biologi; Genetics; cytogenetics; Genetik; cytogenetik;

    Abstract : Telomeres are specialized complexes of DNA and associated proteins that protect the ends of eukaryotic chromosomes. Although most organisms have short repeated DNA sequences at their telomeres, which are specified and maintained by the enzyme telomerase, there are a few exceptions. READ MORE

  5. 5. Expression profiling using manifold supports

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Anette Hagberg; Uppsala universitet.; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; mRNA; expression; minimal residual disease MRD ; chronic myeloid leukemia CML ; recombinant human erythropoietin r-HuEpo ; anemia; insulin dependent diabetes mellitus IDDM ; transplantation; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Analyses of mRNA provides a condensed view of gene structure and quantitative analysis can reveal the induction of physiological or pathological gene expression programs. This thesis describes a new method for mRNA isolation, followed by sensitive real time detection via polymerase chain reaction (PCR), in order to quantitate transcripts of interest. READ MORE