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Showing result 1 - 5 of 127 swedish dissertations matching the above criteria.

2. 1. HIV-1 reverse transcriptase activity assays based on monoclonal antibodies to native enzyme : Applications on studies of antiviral substances and immunological variation of reverse transcriptase

   Author : Ann-Sofie Rytting; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; HIV-1; reverse transcriptase; monoclonal antibody; activity assay; subtype; RT inhibitor; Genetik; Clinical genetics; Klinisk genetik; Medical Genetics; medicinsk genetik;

   Abstract : Specific detection of HIV-1 reverse transcriptase (RT) activity in crude materials was enhanced using a capture assay for immunopurification and subsequent activity assay. The purification step was achieved by anti-HIV-1 RT monoclonal antibodies (Mabs) immobilized onto a solid carrier. READ MORE

4. 2. Reverse Transcriptase Activity Assays for Retrovirus Quantitation and Characterization

   Author : Anders Malmsten; Ulf Gyllensten; Clas Källander; Lynn Morris; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicine; retrovirus; reverse transcriptase; enzyme activity assay; MuLV; HIV; RT purification; viral load; drug susceptibility; Medicin; Dermatology and venerology; clinical genetics; internal medicine; Dermatologi och venerologi; klinisk genetik; invärtesmedicin;

   Abstract : Reverse transcriptase (RT) is a crucial enzyme for retrovirus replication, and its presence in the virion is indispensable for infectivity. This thesis illustrates the use of RT activity assays as tools for quantitation and characterization of different retroviruses, particularly HIV. READ MORE

5. 3. Structure-aided design of antiviral drugs : Application of the method on HIV-1 protease and SIV reverse transcriptase

   Author : Hans Ola Andersson; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Developmental biology; HIV; protease; SIV; reverse transcriptase; structure; inhibitor; drug design; Utvecklingsbiologi; Developmental biology; Utvecklingsbiologi; molekylärbiologi; Molecular Biology;

   Abstract : Many efforts have been made to control the AIDS epidemic. Extensive studies have been done on the biology, biochemistry, and structural biology of HIV in the search for antiviral drugs. The viral-encoded enzymes reverse transcriptase and protease have been main targets for drug design. READ MORE

6. 4. Studies of Retroviral Reverse Transcriptase and Flaviviral Protease Enzymes as Antiviral Drug Targets : Applications in Antiviral Drug Discovery & Therapy

   Author : Muhammad Junaid; Jarl ES Wikberg; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Virus; enzymes; HIV AIDS; retroviral reverse transcriptase; flaviviral protease; NRTIs; proteochemometrics; drug resistance; DEN; JEV; NS2B H -NS3pro; antiviral; drug targets; drug discovery; drug therapy.; Farmakologi; Pharmacology;

   Abstract : Viruses are a major threat to humans due to their unique adaptability, evolvability and  capability to control their hosts as parasites and genetic elements. HIV/AIDS is the third largest cause of death by infectious diseases in the world, and drug resistance due to the viral mutations is still the leading cause of treatment failure. READ MORE

7. 5. Assays for reverse transcriptase activity and p53 protein : applications for characterisation of HIV RT mutants and inhibitors and for p53 expression in breast cancer

   Author : Johan Lennerstrand; Uppsala universitet; []
   Keywords : MEDICINE; MEDICIN;

   Abstract : .... READ MORE