Search for dissertations about: "rolling circle amplification"

Showing result 1 - 5 of 37 swedish dissertations containing the words rolling circle amplification.

  1. 1. Padlock Probes and Rolling Circle Amplification New Possibilities for Sensitive Gene Detection

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Maritha Mendel-Hartvig; Uppsala universitet.; [2002]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probes; RCR; in situ hybridization; branched DNA; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : A series of novel methods for detection of known sequence variants in DNA, in particular single nucleotide polymorphism, using padlock probes and rolling circle replication are presented. DNA probes that can be circularized – padlock probes – are ideal for rolling circle replication. READ MORE

  2. 2. Genetic Analyses using Rolling Circle or PCR Amplified Padlock Probes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Johan Banér; Uppsala universitet.; [2003]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Padlock probes; rolling circle amplification; PCR; genotyping; microarray; expression analysis; Molekylärmedicin; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Internal medicine Molecular medicine genetics and pathology ; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Invärtesmedicin Molekylär medicin genetik och patologi ; Medical Genetics; medicinsk genetik;

    Abstract : Padlock probes are useful in a variety of genetic applications, some of which require that the probes are amplified in order to generate detectable signals. Two general padlock amplification methods, RCA and PCR, are discussed in this thesis. READ MORE

  3. 3. Detection and Sequencing of Amplified Single Molecules

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Rongqin Ke; Uppsala universitet.; [2012]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE

  4. 4. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Olle Ericsson; Uppsala universitet.; [2008]
    Keywords : Molecular medicine; Microarray; proximity ligation; Padlock probe; Rolling circle amplification; Splicing; single molecule; Molekylärmedicin;

    Abstract : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. READ MORE

  5. 5. Development of Electrical Readouts for Amplified Single Molecule Detection

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Camilla Russell; Uppsala universitet.; [2015]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; metallization; electrical DNA detection; magnetic nanoparticles; dielectrophoresis; boranephosphonate modified nucleotides; DNA elongation; Biokemi; Biochemistry;

    Abstract : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. READ MORE