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Showing result 16 - 20 of 46 swedish dissertations matching the above criteria.

2. 16. Nucleic acid analysis tools : Novel technologies and biomedical applications

   Author : Iván Hernández-Neuta; Mats Nilsson; Frank Bier; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Nucleic acid analysis; padlock probes; selector probes; rolling circle amplification; microfluidics; single-molecule detection; digital quantification; fluorescence microscopy; mobile phone microscopy; molecular diagnostics; virology; Biochemistry; biokemi;

   Abstract : Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. READ MORE

4. 17. In situ Sequencing : Methods for spatially-resolved transcriptome analysis

   Author : Marco Mignardi; Mats Nilsson; Xiaoliang Sunney Xie; Stockholms universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Padlock probes; rolling circle amplification; in situ sequencing; spatially-resolved transcriptomics; molecular diagnostics; Biochemistry; biokemi;

   Abstract : It is well known that cells in tissues display a large heterogeneity in gene expression due to differences in cell lineage origin and variation in the local environment at different sites in the tissue, a heterogeneity that is difficult to study by analyzing bulk RNA extracts from tissue. Recently, genome-wide transcriptome analysis technologies have enabled the analysis of this variation with single-cell resolution. READ MORE

5. 18. Molecular Diagnostics Using Volume-Amplified Magnetic Nanobeads : Towards the Development of a Novel Biosensor System

   Author : Mattias Strömberg; Maria Strømme; Peter Svedlindh; Mikkel Fougt Hansen; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Magnetic Biosensors; Ferrofluid; Biofunctionalisation; Ferrofluidic Aging; Interbead Interactions; Magnetic Nanobeads; Complex Magnetisation; Brownian Relaxation; Padlock Probes; Rolling Circle Amplification; Engineering physics; Teknisk fysik;

   Abstract : Micro- or nanometer sized magnetic particles (beads) currently have a vast range of life science applications in, for example, bioseparation techniques, cancer therapy, development of contrast agents and biosensing techniques. In the latter field, magnetic beads offer several unique advantages, including minimal background signals, physical and chemical stability and low manufacturing costs. READ MORE

6. 19. Molecular Tools for Biomarker Detection

   Author : Lei Chen; Ulf Landegren; Niko Hildebrandt; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Rolling circle amplification; padlock probe;

   Abstract : The advance of biological research promotes the emerging of new methods and solutions to answer the biological questions. This thesis describes several new molecular tools and their applications for the detection of genomic and proteomic information with extremely high sensitivity and specificity or simplify such detection procedures without compromising the performance. READ MORE

7. 20. Digital Electrical DNA Sensing

   Author : Maoxiang Guo; Wouter van der Wijngaart; Fredrik Westerlund; KTH; []
   Keywords : DNA detection; thiol-ene-epoxy; rolling circle amplification; digital measurement; DNA stretching; microwell-array; gold nanowires; electrical measurement; specificity; limit of detection; liquid storage; disease diagnosis; lab-on-a-chip; point-of-care.;

   Abstract : Molecule detection is a workhorse in life sciences and medicine, for example in cancer diagnosis and virus and bacterial detection. DNA analysis can provide vital information about the state of a host organism and its medical and health condition. READ MORE