Search for dissertations about: "rolling circle amplification"
Showing result 6 - 10 of 46 swedish dissertations containing the words rolling circle amplification.
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6. Detection and Sequencing of Amplified Single Molecules
Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE
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7. Biomolecular Analysis by Dual-Tag Microarrays and Single Molecule Amplification
Abstract : Padlock probes and proximity ligation are two powerful molecular tools for detection of nucleic acids and proteins, respectively. Both methods result in the formation of DNA reporter molecules upon recognition of specific target molecules. READ MORE
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8. Development of Electrical Readouts for Amplified Single Molecule Detection
Abstract : Molecular diagnostics is a fast growing field with new technologies being developed constantly. There is a demand for more sophisticated molecular tools able to detect a multitude of molecules on a single molecule level with high specificity, able to distinguish them from other similar molecules. READ MORE
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9. Application of Padlock Probe Based Nucleic Acid Analysis In Situ
Abstract : The great variation displayed by nucleic acid molecules in human cells, and the continuous discovery of their impact on life, consequently require continuous refinements of molecular analysis techniques. Padlock probes and rolling circle amplification offer single nucleotide discrimination in situ, a high signal-to-noise ratio and localized detection within cells and tissues. READ MORE
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10. Single Molecule Detection : Microfluidic Automation and Digital Quantification
Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE