Search for dissertations about: "selective real-time PCR"

Showing result 1 - 5 of 13 swedish dissertations containing the words selective real-time PCR.

2. 1. Endogenous Retroviral RNA Expression in Humans

   Author : Lijuan Hu; Jonas Blomberg; Göran Friman; Francois Mallet; Uppsala universitet; []
   Keywords : Microbiology; Human endogenous retrovirus HERV ; HERVE; HERVW; betaretrovirus; gammaretrovirus; RNA expression; real time PCR; endometrium; endometriosis; brain tissue; reproductive tissue; ovarian cancer; ovary; neuroblastoma cell line; Mikrobiologi;

   Abstract : Human endogenous retroviruses (HERVs) constitute about 8% of the human genome. There are around 4000 pol-containing retroviral integrations in the human genome, which makes it impractical to measure each of them separately. READ MORE

4. 2. Expression and Regulation of P2 Receptors in Human Circulation

   Author : Lingwei Wang; Sektion II; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Western blotting; Cardiovascular system; Kardiovaskulära systemet; vascular remodeling; systemic lupus erythematosus; smooth muscle cell; shear stress; real-time PCR; platelets; P2 receptor; gene expression; erythrocyte; ATP release; endothelium;

   Abstract : P2 receptors mediate the actions of the extracellular nucleotides ATP, ADP, UTP and UDP regulating several physiological responses including cardiac function, vascular tone, smooth muscle cell (SMC) proliferation, platelet aggregation, and the release of endothelial factors. The aim of the present study was to investigate the mRNA and protein expression of P2 receptors in human SMCs, endothelial cells (ECs), erythrocytes and platelets, the regulation of P2 receptors under shear stress in human vessels, the changes of platelet P2 receptors in patients with systemic lupus erythematosus (SLE) and the regulation of plasma ATP levels by P2 receptor on erythrocytes. READ MORE

5. 3. Detection and significance of drug-resistant HIV-1 minor populations

   Author : Tobias Bergroth; Karolinska Institutet; Karolinska Institutet; []
   Keywords : HIV-1; antiretroviral treatment; resistance; M184I V; minor populations; selective real-time PCR;

   Abstract : The human immunodeficiency virus (HIV) is the cause of the HIV/AIDS pandemic affecting millions of people around the world. Treatment with antiretroviral drugs, if used properly, leads to a dramatic decrease in virus production in the patient and prevents the onset of AIDS. READ MORE

6. 4. Antibiotic Resistance in Wastewater : Methicillin-resistant Staphylococcus aureus (MRSA)and antibiotic resistance genes

   Author : Stefan Börjesson; Per-Eric Lindgren; Johanna Ericson Sollid; Linköpings universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; MRSA; SCCmec; spa typing; Staphylococcus aureus; methicillin; B-lactam; aminoglycoside; tetracycline; antibiotic; wastewater; wastewater treatment plant; Microbiology; Mikrobiologi;

   Abstract : A large part of the antibiotics consumed ends up in wastewater, and in the wastewater the antibiotics may exert selective pressure for or maintain resistance among microorganisms. Antibiotic resistant bacteria and genes encoding antibiotic resistance are commonly detected in wastewater, often at higher rates and concentrations compared to surface water. READ MORE

7. 5. Molecular epidemiology of acute respiratory virus infections

   Author : Robert Dyrdak; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Acute respiratory virus infections are very common but can also cause severe disease. In my thesis, I have analysed the molecular epidemiology of acute respiratory virus infections caused by enterovirus D68 and coronaviruses. In Paper I, we used real-time PCR and Sanger sequencing to analyse the outbreak of enterovirus D68 in Stockholm in 2016. READ MORE