Search for dissertations about: "selector probes"

Showing result 1 - 5 of 7 swedish dissertations containing the words selector probes.

2. 1. Selector Technology : For Multiplex DNA Analysis

   Author : Fredrik Dahl; Mats Nilsson; Ulf Landegren; Ivo Glynne Gut; Uppsala universitet; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Genetic engineering; DNA technology; Genteknik; Genetic engineering including functional genomics; Genteknik inkl. funktionsgenomik;

   Abstract : A majority of methods for identifying sequences in the human genome involve target sequence amplification through PCR. This work presents novel methods for amplifying circularized DNA and presents solutions for some major limitations of PCR. READ MORE

4. 2. Extracting Genomic Variations using Selector Technology

   Author : Magnus Isaksson; Mats Nilsson; Radoje Drmanac; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Selector; Selector probe; Genetic variation; Resequencing; Targeted sequencing; copy-number variations; MLGA; Bioinformatics; Next generation sequencing; NGS; Amplified single molecule; ASM; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Genetics; Genetik; Molekylär medicin; Molecular Medicine;

   Abstract : This thesis describes the development and use of a new class of molecular tools called Selector probes, and its potential for investigations of genetic variation. The Selector technology provides multiplex amplification of targeted DNA sequences with a high specificity, and an enrichment factor in the same order of magnitude as PCR. READ MORE

5. 3. Nucleic acid analysis tools : Novel technologies and biomedical applications

   Author : Iván Hernández-Neuta; Mats Nilsson; Frank Bier; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Nucleic acid analysis; padlock probes; selector probes; rolling circle amplification; microfluidics; single-molecule detection; digital quantification; fluorescence microscopy; mobile phone microscopy; molecular diagnostics; virology; Biochemistry; biokemi;

   Abstract : Nucleic acids are fundamental molecules of living organisms functioning essentially as the molecular information carriers of life. From how an organism is built to how it responds to external conditions, all of it, can be found in the form of nucleic acid sequences inside every single cell of every life form on earth. READ MORE

6. 4. Development of new tools for multiplex genetic analyses

   Author : Johan Stenberg; Uppsala universitet; []
   Keywords : ;

   Abstract : Several methods allowing the simultaneous interrogation of multiple qualities of nucleic acid samples have been developed recently. Although diverse in nature, many of these methods have common limitations. READ MORE

7. 5. Software Tools for Design of Reagents for Multiplex Genetic Analyses

   Author : Johan Stenberg; Mats Nilsson; Ulf Landegren; Anthony Brookes; Uppsala universitet; []
   Keywords : Bioinformatics; Bioinformatik;

   Abstract : Methods using oligonucleotide probes are powerful tools for the analysis of nucleic acids. During recent years, many such methods have been developed that enable the simultaneous interrogation of multiple qualities of a sample. Many of these multiplexing techniques share common limitations. READ MORE