Search for dissertations about: "semi-rational design"

Found 4 swedish dissertations containing the words semi-rational design.

  2. 1. Protein Engineering of Candida antarctica Lipase A : Enhancing Enzyme Properties by Evolutionary and Semi-Rational Methods

    Author : Anders G. Sandström; Jan-Erling Bäckvall; Uwe T. Bornscheuer; Stockholms universitet; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; lipase; protein engineering; directed evolution; kinetic resolution; structural biology; Enzyme engineering; Enzymteknik; organisk kemi; Organic Chemistry;

    Abstract : Enzymes are gaining increasing importance as catalysts for selective transformations in organic synthetic chemistry. The engineering and design of enzymes is a developing, growing research field that is employed in biocatalysis. READ MORE

  4. 2. Directed Evolution of Glutathione Transferases with Altered Substrate Selectivity Profiles : A Laboratory Evolution Study Shedding Light on the Multidimensional Nature of Epistasis

    Author : Wei Zhang; Bengt Mannervik; Bengt-Harald Jonsson; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; glutathione transferase; azathioprine; directed evolution; semi-rational design; catalytic mechanism; saturation mutagenesis; reduced amino acid alphabets; molecular docking; protein evolution; multivariate data analysis; epistasis; fitness landscape; evolutionary trajectories; Biochemistry; Biokemi; Biokemi; Biochemistry;

    Abstract : Directed evolution is generally regarded as a useful approach in protein engineering. By subjecting members of a mutant library to the power of Darwinian evolution, desired protein properties are obtained. Numerous reports have appeared in the literature showing the success of tailoring proteins for various applications by this method. READ MORE

  5. 3. In silico protein design for the enhancement of protein stability and function

    Author : David A. Hueting; Per-Olof Syrén; Hjalmar Brismar; Zbynek Prokop; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein Engineering; Ancestral sequence reconstruction; terpene cyclases; SARS-CoV-2; Spike protein; PET; PETase; rational engineering; Proteinteknik; Förfäders sekvensrekonstruktion; Terpencyklas; SARS-CoV-2; Spike protein; PET; PETas; rationell proteinteknik; Kemi; Chemistry;

    Abstract : Enzymes are natures catalysts that increase the rate of a chemical reaction. The increased rate of a reaction is required to be able to sustain life. Despite the huge impact of enzymes, they are not perfect catalysts. READ MORE

  6. 4. Structure Function Relationships in Pyrimidine Degrading and Biocatalytic Enzymes, and Their Implications for Cancer Therapy and Green Chemistry

    Author : Dirk Maurer; Doreen Dobritzsch; Mikael Widersten; Rikkert Wierenga; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; β-Ureidopropionase; Dihydropyrimidine Dehydrogenase; Alcohol Dehydrogenase; Pyrimidine catabolism; 5-Fluorouracil; CASTing; X-ray Crystallography;

    Abstract : This thesis includes the work of two separate projects, studies on pyrimidine degrading enzymes and studies on in vitro evolved enzymes. The common denominator of both projects was the use of structural information to explain functional effects, observed in the studied biocatalysts. READ MORE