Search for dissertations about: "siRNA delivery"

Showing result 1 - 5 of 26 swedish dissertations containing the words siRNA delivery.

  2. 1. Chimeric gene delivery vectors : Design, synthesis, and mechanisms from transcriptomics analysis

    Author : Moataz Dowaidar; Ülo Langel; Sandrine Sagan; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell-penetrating peptides; magnetic nanoparticles; graphene oxide; autophagy; siRNA; SCO; QSAR; neurokemi med molekylär neurobiologi; Neurochemistry with Molecular Neurobiology;

    Abstract : Delivery of nucleic acid is a promising approach for genetic diseases/disorders. However, gene therapy using oligonucleotides (ONs) suffers from low transfection efficacy due to negative charges, weak cellular permeability, and enzymatic degradation. READ MORE

  4. 2. Linear and Branched Chitosan Oligomers as Delivery Systems for pDNA and siRNA In Vitro and In Vivo

    Author : Mohamed Mahmoud Issa; Per Artursson; Arto Urtti; Uppsala universitet; []
    Keywords : Medical sciences; Chitosan oligomers; gene delivery; gene expression; pDNA; siRNA; MEDICIN OCH VÅRD;

    Abstract : In this thesis, chitosan, a biocompatible polysaccharide that has been approved as a food additive was selected as a platform for the development of safe, efficient non-viral gene delivery systems to mammalian cells. Previously, chitosan-based gene formulations had been generally associated with high molecular weight chitosans, which were poorly characterised in terms of molecular weight distribution and degree of acetylation. READ MORE

  5. 3. miRNA and Asymmetric siRNA : Small RNAs with Large Effects on Bone Metabolism

    Author : Navya Laxman; Andreas Kindmark; Sara Windahl; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; miRNA; cp-siRNA; RNAi; bone; Osteoblast; Sequencing; Differential expression; Wnt pathway; Biology with specialization in Molecular Biology; Biologi med inriktning mot molekylärbiologi;

    Abstract : RNA interference (RNAi) is a post-transcriptional gene silencing process elicited by double-stranded RNA, such as micro-RNA (miRNA) and small interfering RNA (siRNA). They are 18-25 nucleotide long, small non-coding RNAs acting as critical regulators in eukaryotic genome expression. READ MORE

  6. 4. Vectorization of oligonucleotides with cell-penetrating peptides : Characterization of uptake mechanisms and cytotoxicity

    Author : Samir EL Andaloussi; Ülo Langel; Alain Prochiantz; Stockholms universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; CPP; endocytosis; splice correction; siRNA; PNA; cargo delivery; M918; Neurochemistry; Neurokemi; Neurochemistry and Molecular Neurobiology; neurokemi med molekylär neurobiologi;

    Abstract : The hydrophobic plasma membrane constitutes an indispensable barrier for cells in living animals. Albeit being pivotal for the maintenance of cells, the inability to cross the plasma membrane is still one of the major obstacles to overcome in order to progress current drug development. READ MORE

  7. 5. Detection and quantification of lipid-mediated siRNA delivery to the cytosol

    Author : Hampus Hedlund; Tumörmikromiljö; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; RNA Interference; RNA Therapeutics; Endosomal Escape; Lipid Mediated Delivery; Live-Cell Imaging;

    Abstract : Cancer is one of the leading causes of death globally. Novel therapeutics is urgently needed to advance treatment, especially for generalized disease where current treatment options have a poor prognosis. In particular, targeted therapy that can address genetic changes would be of great value. READ MORE