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Showing result 1 - 5 of 21 swedish dissertations matching the above criteria.

  2. 1. Computational Modelling of Ligand Complexes with G-Protein Coupled Receptors, Ion Channels and Enzymes

    Author : Lars Boukharta; Johan Åqvist; Christopher A. Reynolds; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; computer simulations; molecular dynamics; ligand binding; free energy perturbation; linear interaction energy; binding free energy; homology modelling; structure prediction; alanine scanning; site-directed mutagenesis; hERG; GPCR; neuropeptide Y; HIV-1 reverse transcriptase; integron integrase; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Accurate predictions of binding free energies from computer simulations are an invaluable resource for understanding biochemical processes and drug action. The primary aim of the work described in the thesis was to predict and understand ligand binding to several proteins of major pharmaceutical importance using computational methods. READ MORE

  4. 2. Functional mapping and in vivo metabolism of the monoclonal antibody TS1 and its single-chain fragment : Its interaction with the antigen and the anti-idiotype

    Author : Patrik Holm; Birgitta Sundström; Lars Gedda; Karlstads universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single-chain antibody; functional mapping; site-directed mutagenesis; immune complex; metabolism; Molecular biology; Molekylärbiologi; Biomedical Laboratory Science; Biomedicinsk laboratorievetenskap;

    Abstract : Antibodies are proteins capable of specific interactions to a wide range of molecules. These interactions are facilitated by the complementary determining regions (CDR).Carcinomas are the most common of human cancers and they release significant amount of cytokeratins (CK) in the necrotic areas of the tumors. READ MORE

  5. 3. Thermostable Phytase from a Bacillus sp.: Heterologous Production, Mutation, Characterization and Assay Development

    Author : Tran Thi Thuy; Bioteknik; []
    Keywords : ß-propeller phytase; recombinant protein; site-directed mutagenesis; phytate;

    Abstract : Phytase is an important enzyme in the food/feed industry. It catalyzes the hydrolysis of phytate, an anti-nutrient compound present in cereals and grains, to release orthophosphate and myo-inositol-6-phosphate with lower degrees of phosphorylation. READ MORE

  6. 4. Leukotriene A4 hydrolase : studies of structure-function relationships by site-directed mutagenesis and X-ray crystallography

    Author : Peter C Rudberg; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Inflammation is the pathophysiological mechanism by which an organism clears and rebuilds a site of infection or injury. It is also the characteristic feature of a multitude of severe diseases, with the potential of afflicting almost any organ system. READ MORE

  7. 5. Microsomal glutathione transferase : catalysis, in vitro mutagenesis and heterologous expression

    Author : Rolf Weinander; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Microsomal glutathione transferase is a membrane-bound member of the glutathione transferases, a family of multifunctional enzymes involved in the cellular detoxification of xenobiotics and reactive endogenous compounds formed during oxidative stress. Rat liver microsomal glutathione transferase is a trimeric protein with a molecular mass of 17. READ MORE