Search for dissertations about: "side directed mutagenesis"

Showing result 11 - 15 of 21 swedish dissertations containing the words side directed mutagenesis.

  2. 11. X-ray structure analysis of short-chain dehydrogenases/reductases

    Author : Jordi Benach-Andreu; Karolinska Institutet; Karolinska Institutet; []
    Keywords : crystal structure; short-chain dehydrogenases reductases; Drosophila alcohol dehydrogenase; catalytic reaction mechanism; Comamonas testosteroni 3[beta] 17[beta]hydroxysteroid dehydrogenase;

    Abstract : X-ray crystallography and site-directed mutagenesis were used to better understand the structure/function relationships in the family of short-chain dehydrogenases/reductases (SDR). This group of enzymes constitutes a protein family with at least 60 members with highly diverse functions in pro- and eukaryotes. READ MORE

  4. 12. Interaction studies of idiotypic and antiidiotypic antibodies at experimental tumor targeting

    Author : Ann Erlandsson; Mats Ohlin; Umeå universitet; []
    Keywords : anti-idiotypic antibody; clearing; cytokeratin 8; epitope; idiotypic antibody; immunotherapy;

    Abstract : An increasing number of antibodies are being labeled with radionuclides for immunotargeting, but the negative side effects generated on normal tissues by the nuclide remains a significant problem. The clearing of non-tumor-targeted radiolabeled antibodies from the circulation contributes significantly to improved targeting; it has been demonstrated that anti-idiotypic antibodies can be used for this purpose. READ MORE

  5. 13. Beta-Glycosidases from Hyperthermophiles as Biocatalysts

    Author : Therese Hansson; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Bioteknik; Biochemical technology; Biokemisk teknik; Proteins; enzymology; Proteiner; enzymologi; Biotechnology; high temperatures; water activity; activity; selectivity; hexanol; hexyl-b-glycoside; galacto-oligosaccharides; lactose; hyperthermostable b-glycosidase; hydrolysis; optimisation; transglycosylation;

    Abstract : The thesis concerns glycosyl transfer with use of b-glycosidases, most of them from hyperthermophilic organisms. Both optimisation of the reaction conditions and protein engineering in terms of site-directed mutagenesis were used to improve b-glycosidase-catalysed reactions. READ MORE

  6. 14. A1M: a heme and radical binding protein. A study on structure, function and mechanisms

    Author : Sigurbjörg Rutardottir; Infektionsmedicin; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; α1-microglobulin; oxidative stress; heme; radiation; reactive oxygen species; preeclampsia;

    Abstract : This thesis describes functional and structural studies of the human protein α1-microglobulin (A1M), an evolutionarily well-conserved 26 kDa plasma and tissue protein mainly synthesized in the liver. A1M belongs to the lipocalin protein family. Lipocalins share a very similar structur, an 8-stranded β-barrel forming a hydrophobic pocket. READ MORE

  7. 15. Regulation of tartrate-resistant purple acid phosphatase by proteolytic processing in rat

    Author : Jenny Ljusberg-Sjölander; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Purple acid phosphatase; enzyme catalysis; proteolytic processing;

    Abstract : Purple acid phosphatase (PAP) is an iron-containing binuclear acid metallohydrolase with a characteristic purple colour, also referred to as tartrate-resistant acid phosphatase (TRAP), uteroferrin or type 5 acid phosphatase. PAP is highly expressed in osteoclasts and has a role in bone resorption. READ MORE