Search for dissertations about: "side directed mutagenesis"

Showing result 6 - 10 of 21 swedish dissertations containing the words side directed mutagenesis.

  2. 6. Short-chain dehydrogenases/reductases : structure, function and motifs of hydroxysteroid dehydrogenases

    Author : Charlotta Filling; Karolinska Institutet; Karolinska Institutet; []
    Keywords : Short-chain dehydrogenases reductases; site-directed mutagenesis; steady-state kinetics; X-ray structure; enzyme function; steroid; motif; reaction mechanism; 3 beta 17 beta-hydroxysteroid dehydrogenase; 17 beta-hydroxysteroid dehydrogenase; short chain hydroxyacyl coenzyme A dehydrogenase;

    Abstract : Short-chain dehydrogenases/reductases (SDR) constitute a superfamily of proteins catalysing reactions of a wide range of substrates in all life forms. During the past years, a large number of new proteins belonging to this superfamily has been detected. They are important in e.g. READ MORE

  4. 7. Mechanistic insights into the biosynthesis of polyketide antibiotics

    Author : Azmiri Sultana; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : Anthracyclines are a group of aromatic polyketide compounds with significant medical importance due to their antineoplastic properties. Doxorubicin and daunorubicin members of this family are among the two most commonly used anticancer drugs. However, these compounds exhibit severe side effects like cardiotoxicity and multi-drug resistance. READ MORE

  5. 8. Poly(A)-Specific Ribonuclease (PARN)

    Author : Yan-Guo Ren; Eric Westhof; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; Poly A -specific ribonuclease; PARN; deadenylation; processive; oligomeric; cap interacting; aminoglycoside; active site; FE2 -mediated cleavage; metal ion switch; Cell- och molekylärbiologi; Cell and molecular biology; Cell- och molekylärbiologi; Molekylär cellbiologi; Molecular Cellbiology;

    Abstract : Degradation of the mRNA 3'-end located poly(A) tail is an important step for mRNA decay in mammalian cells. Thus, to understand mRNA decay in detail, it is important to identify the catalytic activities involved in degrading poly(A). READ MORE

  6. 9. Hydrolases as Catalysts for Green Chemistry and Industrial Applications - Esterase, Lipase and Phytase

    Author : Yasser Gaber; Bioteknik; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; HPLC-EAT; sustainable environment; mutation; metallo-enzyme; phytate; b-propeller; biodegraqdable; metrics; green chemistry; Novozym 435; CalB; solvent-free; kinetic resolution; Biocatalysis; rational design; PLE; esterase; clopidogrel; enantioselectivity;

    Abstract : The use of enzymes in industrial applications has been recognised for providing clean processes with minimal impact on the environment. This thesis presents studies on engineering of enzymes and enzyme-based processes in the light of green chemistry and environmental sustainability, and focuses on three hydrolases: esterase, lipase and phytase. READ MORE

  7. 10. A Chloroplast Localized Small Heat Shock Protein, Hsp21. Importance of Hsp21 in stress protection of transgenic Arabidopsis thaliana, recombinant expression and characterization of oxidation-dependent conformational changes

    Author : Ulrika Härndahl; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; a-crystallin; sHsp; chaperone; amphipathic a-helix; oligomer; redox-regulated; methionine sulfoxidation; oxidative stress; regulation; protein structure; circular dichroism; mass spectrometry; fluorescence spectroscopy; Biochemistry; Metabolism; Biokemi;

    Abstract : A specific group of heat shock proteins, the so called small heat shock proteins (sHsps), exists in all organisms but are especially abundant in plants. The sHsps prevent aggregation of other proteins (substrate proteins) during transient heat and oxidative stress. READ MORE