Search for dissertations about: "single cell PCR"

Showing result 1 - 5 of 120 swedish dissertations containing the words single cell PCR.

  2. 1. Droplet microfluidics for single cell and nucleic acid analysis

    Author : Prem Kumar Periyannan Rajeswari; Helene Andersson Svahn; Håkan Jönsson; Martin Dufva; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Acoustophoresis; Biomarker detection; Cell behavior analysis; Cell factories; Droplet microfluidics; Droplet PCR; High throughput biology; Label-free enrichment; Single cell analysis; Bioteknologi; Biotechnology;

    Abstract : Droplet microfluidics is an emerging technology for analysis of single cells and biomolecules at high throughput. The controlled encapsulation of particles along with the surrounding microenvironment in discrete droplets, which acts as miniaturized reaction vessels, allows millions of particles to be screened in parallel. READ MORE

  4. 2. Giant cell arteritis. Epidemiological, morphological and molecular genetic studies

    Author : Vigdís Pétursdóttir; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Giant cell arteritis; temporal artery; aorta; epidemiology; atrophy; calcification; estrogen receptor a; morphometry; immunocytochemistry; Western blot; molecular genetics;

    Abstract : Giant cell arteritis (GCA) is a chronic inflammatory disorder of unknown etiology, affecting large and medium-sized arteries, predominantly in postmenopausal women. Its pathogenesis is probably multifactorial. Several studies suggest that GCA is an antigen-driven disease. READ MORE

  5. 3. Droplet Microfluidics reverse transcription and PCR towards Single Cell and Exosome Analysis

    Author : Lovisa Söderberg; Helene Andersson Svahn; Håkan Jönsson; Valérie Taly; KTH; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Droplet microfluidics; Reverse transcription; Droplet PCR; High Throughput biology; Single cell Analysis; Exosomes; Bioteknologi; Biotechnology;

    Abstract : Miniaturization of biological analysis is a trend in the field of biotechnology aiming to increase resolution and sensitivity in biological assays. Decreasing the reaction volumes to analyze fewer analytes in each reaction vessel enables the detection of rare analytes in a vast background of more common variants. READ MORE

  6. 4. Detection and Sequencing of Amplified Single Molecules

    Author : Rongqin Ke; Mats Nilsson; Ulf Landegren; Radoje Drmanac; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE

  7. 5. Quantification of gene expression in single cells

    Author : Martin Bengtsson; Islet cell physiology; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Medicin människa och djur ; Medicine human and vertebrates ; Biomedical sciences; Biomedicinska vetenskaper; quantitative gene expression analysis; single cell PCR; islets of Langerhans;

    Abstract : Studies of apparently homogeneous cell populations and single cells often give highly divergent results. Cells exhibit varying responsiveness to stimuli and gene expression and they are in many aspects stochastic and unpredictable. We have developed a method to measure gene expression quantitatively in individual cells with real-time RT-PCR. READ MORE