Search for dissertations about: "single chain variable fragments"

Showing result 1 - 5 of 10 swedish dissertations containing the words single chain variable fragments.

2. 1. Construction, expression and evaluation of anti-keratin 8 single-chain antibody fragments

   Author : Rozbeh Jafari; Birgitta Sundström; Jörgen Carlsson; Karlstads universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Single-chain antibody fragments; keratin 8; site-directed mutagenesis; heterologous expression; P. pastoris; design of experiments; multicellular tumor spheroids; immunotargeting; Biomedical Sciences; Biomedicinsk vetenskap;

   Abstract : Antibodies are glycoproteins specifically binding to a variety of antigens and today extensively used in prevention, diagnosis and treatment of diseases. Carcinomas originate from epithelial tissues and are the most common forms of human malignancies. In the necrotic areas of carcinomas significant amounts of keratins (K) are found extracellularly. READ MORE

4. 2. Functionalization of spider silk with affinity and bioactive domains via genetic engineering for in vitro disease diagnosis and tissue engineering

   Author : Naresh Thatikonda; My Hedhammar; Hanna Dams Kozlowska; KTH; []
   Keywords : affinity domains; artificial ECM; basic fibroblast growth factor; functionalization; mammalian cell culture; micro-and nano arrays; partial spider silk; single chain variable fragments; Bioteknologi; Biotechnology;

   Abstract : In the recent past, spider silk has drawn significant attention from researchers mainly due to its distinguished mechanical strength, elasticity, biocompatibility and biodegradability. Technological advancements in genetic engineering have resulted in methods for creation of partial spider silk proteins. READ MORE

5. 3. Calcium-dependent Affinity Domains for the Purification of Antibodies and Antibody Fragments

   Author : Julia Scheffel; Sophia Hober; Alois Jungbauer; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Antibodies; Antibody fragments; Biomanufacturing; Protein purification; Protein engineering; Protein A; ZCa; Calcium; Mild elution; Bioteknologi; Biotechnology;

   Abstract : Antibodies are essential proteins in both our bodies and biotechnological research, and hold outstanding therapeutic value. The market for antibody-based therapeutics has grown exponentially during the last decades, owing to several advantages over small molecule drugs, such as fewer undesirable side effects associated with a higher target specificity. READ MORE

6. 4. Lactobacilli expressing antibody fragments against pathogens

   Author : Anna Hultberg; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Adherence to mucosal surfaces has been shown to be of great importance in the pathogenesis of various infections in the gastro-intestinal tract. Live recombinant bacteria can be used to deliver active or passive immunity at the mucosal surface, which is the site of entry for the majority of the pathogens. READ MORE

7. 5. Antibody-Fragments: Protein Engineering, Process Development, and their Application as Affinity Ligands

   Author : Laila Sakhnini; Tillämpad biokemi; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Monoclonal Antibodies; Aggregation; Biophysical Characterisation; Process Development; Affinity Adsorbents; Monoclonal Antibodies; Aggregation; Biophysical Characterisation; Process Development; Affinity Adsorbents;

   Abstract : Monoclonal antibody (mAb) fragments have become the formats of choice for both biopharmaceuticals and ligands in analytical and preparative applications. Their smaller size enables additional advantages over full-length mAbs, such as improved tissue penetration, faster blood clearance for diagnostic applications, improved capacity, and enablement of multivalent formatting. READ MORE