Search for dissertations about: "single molecule sensing"

Showing result 1 - 5 of 12 swedish dissertations containing the words single molecule sensing.

  1. 1. Single Molecule Detection Microfluidic Automation and Digital Quantification

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Malte Kühnemund; Uppsala universitet.; [2016]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

  2. 2. From single-molecule sensing to extracellular vesicles in glioma cells under stress

    University dissertation from ; Chalmers tekniska högskola; Gothenburg

    Author : Virginia Claudio; [2017]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteome; single molecule sensing; nanoplasmonics; surface plasmon resonance; exosomes; glioblastoma; extracellular vesicles; cellular stress; lipid composition;

    Abstract : This thesis describes the work I conducted in two different areas, namely biomolecular sensing and quantitative cell biology. The work in the former area was focused on the optimization of plasmonic metamaterials for sensing applications, and in the latter on the characterization of extracellular vesicles released by glioblastoma cells under stress conditions. READ MORE

  3. 3. Detection and Sequencing of Amplified Single Molecules

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Rongqin Ke; Uppsala universitet.; [2012]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; padlock probes; proximity ligation assay; rolling circle amplification; circle-to-circle amplification; single molecule detection; amplified single molecules; sequencing; in situ; single cell; Molecular Biotechnology; Molekylär bioteknik;

    Abstract : Improved analytical methods provide new opportunities for both biological research and medical applications. This thesis describes several novel molecular techniques for nucleic acid and protein analysis based on detection or sequencing of amplified single molecules (ASMs). READ MORE

  4. 4. Miniaturized localized surface plasmon resonance biosensors

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Si Chen; [2013]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; colloidal lithography; Surface plasmon resonance; biosensing; electron beam lithography; single molecule; hyperspectral imaging; photo functionalization;

    Abstract : Abstract Reliable and sensitive biosensors are required for fast and accurate diagnostics. Localized surface plasmon resonances (LSPRs) in noble-metal nanoparticles possess very high refractive index sensitivity close to the metal surface and therefore constitute an attractive biosensing platform. READ MORE

  5. 5. Silicon Nanopore Arrays Fabrication and Applications for DNA Sensing

    University dissertation from KTH Royal Institute of Technology

    Author : Miao Zhang; KTH.; [2018]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; nanopore; array; electrochemical etching; DNA; optics; thermophoresis;

    Abstract : Nanopore biomolecule sensing and sequencing has emerged as a simple but powerful tool for single molecule studies over the past two decades. By elec- trophoretically driving single molecules through a nanometer-sized pore, often sitting in an insulating membrane that separates two buffer solutions, ionic current blockades can be detected to reveal rich information of the molecules, such as DNA length, protein size and conformation, even nucleic acid se- quence. READ MORE