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Showing result 1 - 5 of 430 swedish dissertations matching the above criteria.

2. 1. Single Molecule Detection : Microfluidic Automation and Digital Quantification

   Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

   Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

4. 2. Single molecule dynamics

   Author : Lars Edman; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : Properties found in an ensemble of molecules may not be understood if it is not possible to study each molecule one by one. This thesis is concerned with the study of the dynamic properties of individual complex biological molecules as observed by confocal single-molecule fluorescence spectroscopy. READ MORE

5. 3. Genotyping and Mutation Detection In Situ : Development and application of single-molecule techniques

   Author : Ida Grundberg; Mats Nilsson; Ulf Landegren; Olli-Pekka Kallioniemi; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; padlock probes; in situ; rolling circle amplification; mRNA; genotyping; mutation detection; cancer; tissue sections; diagnostics; single-molecule; single-cell; microscopy; Molecular medicine genetics and pathology ; Molekylär medicin genetik och patologi ; Molekylär medicin; Molecular Medicine;

   Abstract : The human body is composed of trillions of cells closely working together to maintain a functional organism. Every cell is unique in molecular composition and can acquire genetic variations that might cause it to turn pathological. READ MORE

6. 4. Tracking single molecules in uncharted territory : A single-molecule method to study kinetics in live bacteria

   Author : Javier Aguirre Rivera; Magnus Johansson; Dmitri N. Ermolenko; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; single-molecule; protein synthesis; fluorescence microscopy; antibiotics; aminoglycosides; macrolides; apramycin; gentamicin; paromomycin; S13; translation; bacteria; Molekylär biovetenskap; Molecular Life Sciences;

   Abstract : The synthesis of proteins, also known as translation, is a fundamental process in every living organism. The steps in the translation of genetic information to functional proteins have been meticulously studied, mostly using in vitro techniques, yielding a detailed model of their mechanisms. READ MORE

7. 5. Technologies for Single Cell Genome Analysis

   Author : Erik Borgström; Afshin Ahmadian; Sten Linnarsson; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA; sequencing; single molecule; single cell; whole genome amplification; exome sequencing; emulsions; barcoding; phasin; Biotechnology; Bioteknologi;

   Abstract : During the last decade high throughput DNA sequencing of single cells has evolved from an idea to one of the most high profile fields of research. Much of this development has been possible due to the dramatic reduction in costs for massively parallel sequencing. READ MORE