Search for dissertations about: "single particle imaging"

Showing result 1 - 5 of 55 swedish dissertations containing the words single particle imaging.

  2. 1. Structural Studies of Large dsDNA Viruses using Single Particle Methods

    Author : Hemanth Kumar Narayana Reddy; Martin Svenda; Sarah Butcher; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; PR772; phage; PRD1; Bacteriophage; coliphage; Melbournevirus; Cedratvirus; lurbo; Pithovirus; CryoEM; Single particle imaging; Coherent; Diffractive; Imaging; Soft X-ray; Microscopy; Microscope; GDVN; High resolution; XFEL; aerosol; Injection; electrospray; gas dynamic virtual nozzle; CDI; CXI; FEL; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Abstract : Structural studies of large biological assemblies pose a unique problem due to their size, complexity and heterogeneity. Conventional methods like x-ray crystallography, NMR, etc. are limited in their ability to address these issues. To overcome some of these limitations, single particle methods were used. READ MORE

  4. 2. Coherent Diffractive Imaging with X-ray Lasers

    Author : Max Felix Hantke; Filipe R. N. C. Maia; Janos Hajdu; Martin Svenda; Edgar Weckert; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; coherent diffractive X-ray imaging; lensless imaging; coherent X-ray diffractive imaging; flash diffractive imaging; single particle imaging; aerosol injection; electrospray injection; substrate-free sample delivery; carboxysome; phase retrieval; X-ray diffraction software; X-ray free-electron laser; XFEL; FEL; CXI; CDI; CXDI; FXI;

    Abstract : The newly emerging technology of X-ray free-electron lasers (XFELs) has the potential to revolutionise molecular imaging. XFELs generate very intense X-ray pulses and predictions suggest that they may be used for structure determination to atomic resolution even for single molecules. READ MORE

  5. 3. Single Molecule Detection : Microfluidic Automation and Digital Quantification

    Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

  6. 4. Statistical processing of Flash X-ray Imaging of protein complexes

    Author : Alberto Pietrini; Carl Nettelblad; Johan Bielecki; Janos Hajdu; Flavio Capotondi; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; X-ray; XFEL; single particle; hit-finder; statistical hit-finder; single protein; protein complex; RNA polymerase II; FEL; free-electron laser; FXI; Flash X-ray Imaging; CXI; CSPAD; x-ray imaging; COACS; Physics with specialization in Biophysics; Fysik med inriktning mot biofysik;

    Abstract : Flash X-ray Imaging (FXI) at X-ray Free Electron Lasers (XFELs) is a promising technique that permits the investigation of the 3D structure of molecules without the need for crystallization, by diffracting on single individual sample particles.In the past few years, some success has been achieved by using FXI on quite large biological complexes (40 nm-1 μm in diameter size). READ MORE

  7. 5. Imaging Living Cells with an X-ray Laser

    Author : Gijs van der Schot; Tomas Ekeberg; Janos Hajdu; Filipe Maia; Pierre Thibault; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Coherent diffractive X-ray imaging; flash X-ray imaging; lensless imaging; single particle imaging; cyanobacteria; phasing; image classification; substrate-free sample delivery; X-ray free-electron laser; XFEL; FEL; CDXI; CDI; CXI; FXI; Chemistry with specialization in Biophysics; Kemi med inriktning mot biofysik;

    Abstract : Imaging living cells at a resolution higher than the resolution of optical microscopy is a significant challenge. Fluorescence microscopy can achieve a degree of super-resolution via labeling cellular components with a fluorescent dye. READ MORE