Search for dissertations about: "single-molecule biophysics"

Showing result 1 - 5 of 19 swedish dissertations containing the words single-molecule biophysics.

  2. 1. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Author : Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

    Abstract : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. READ MORE

  4. 2. Nanofluidics for Static and Dynamic DNA-Protein Interaction Studies - Repair of Double-Strand Breaks from a Single-Molecule Perspective

    Author : Robin Öz; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Single-molecule; Protein; NHEJ; Dynamic; DNA repair; HR; Biomolecule interactions; Microscopy; Fluorescence; Nanofluidics;

    Abstract : Double-strand breaks (DSBs) is one of the most lethal forms of DNA damage. A single DSB may result in stalling of vital cellular machineries, and thus, requires immediate measures by the cell. READ MORE

  5. 3. From single-molecule sensing to extracellular vesicles in glioma cells under stress

    Author : Virginia Claudio; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; proteome; single molecule sensing; nanoplasmonics; surface plasmon resonance; exosomes; glioblastoma; extracellular vesicles; cellular stress; lipid composition;

    Abstract : This thesis describes the work I conducted in two different areas, namely biomolecular sensing and quantitative cell biology. The work in the former area was focused on the optimization of plasmonic metamaterials for sensing applications, and in the latter on the characterization of extracellular vesicles released by glioblastoma cells under stress conditions. READ MORE

  6. 4. Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

    Author : Joachim Piguet; Horst Vogel; Ruud Hovius; Jerker Widengren; Antoine Triller; Switzerland Ecole Polytechnique Fédérale de Lausanne (EPFL); []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; fluorescence; microscopy; plasma membrane; membrane proteins; membrane proteins diffusion; single-molecule imaging; single-molecule tracking; pentameric ligand-gated ion channels; nicotinic acetylcholine receptor; serotonin receptor; synapse; neuromuscular junction; post-synaptic scaffold; rapsyn; myopathies; fluorescent proteins; Förster resonance energy transfer; FRET imaging; protein-protein interactions; protein trafficking; photo-activatable proteins; Biological Physics; Biologisk fysik;

    Abstract : Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotinic acetylcholine is densely packed under the motor nerve termini. READ MORE

  7. 5. Development of novel bioanalytical assays with single-molecule readout for biomarker detection and drug candidate characterization

    Author : Olov Wahlsten; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; QCM-D; biomarker detection; TIRF microscopy.; Bioanalytical assay; spectrofluorometry; fluorescence; single-molecule readout; drug discovery; liposomes;

    Abstract : Bioanalytical assays with single-molecule readout for studying molecular interactions have in the past decades received increasing attention. The high sensitivity often offered by this readout scheme has for example enabled ultra-sensitive analyte detection, having important implications for monitoring early disease progression and the effects of drug treatment. READ MORE