Search for dissertations about: "single-molecule experiment"

Showing result 1 - 5 of 9 swedish dissertations containing the words single-molecule experiment.

2. 1. How precise is cyclic life? : Insights during a single molecule revolution of the bacterial cell cycle

   Author : Mats Walldén; Johan Elf; Peter Graumann; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; E.coli; cell cycle; replication initiation; transcription factor; gene-regulation; single molecule microscopy; microfluidics; Biology with specialization in Molecular Biotechnology; Biologi med inriktning mot molekylär bioteknik;

   Abstract : Bacterial cells reproduce by doubling in size and dividing. The molecular control systems which regulate the cell cycle must do so in a manner which maintains a similar cell size over many generations. A cell can under conditions of fast growth conclude cell cycles in shorter time than the time required to replicate its chromosome. READ MORE

4. 2. From single-cell transcriptomics to single-molecule counting

   Author : Saiful Islam; Karolinska Institutet; Karolinska Institutet; []
   Keywords : ;

   Abstract : RNA-sequencing (RNA-seq) technology has been progressing so fast in the last few years and made it possible to perform transcriptome analysis at single-cell level that was even unimaginable a few years before. Nowadays, the importance of gene expression analysis at the single-cell level is increasingly appreciated for the study of complex heterogeneous tissue. READ MORE

5. 3. Fluorescence Studies of Membranes -- Proteins and Lipids, their Dynamics and Interactions

   Author : Johan Strömqvist; Jerker Widengren; Thorsten Wohland; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Fluorescence correlation spectroscopy; cytokinesis; collision theory; protein-protein interactions; lipid dynamics; single-molecule experiment; Biological physics; Biologisk fysik;

   Abstract : In this thesis, fluorescence spectroscopy was utilized to probe protein and lipid dynamics and interactions in their native, or close to native, environments. Thereby insight could be gained into the fundamentals of bacterial cell division and the innateimmune system. A particular focus has been devoted to fluorescence fluctuations. READ MORE

6. 4. Towards Single Molecule Imaging - Understanding Structural Transitions Using Ultrafast X-ray Sources and Computer Simulations

   Author : Carl Caleman; Janos Hajdu; Richard Neutze; Uppsala universitet; []
   Keywords : Molecular biophysics; XFEL; Ultrafast Melting; InSb; Molecular Dynamics; Water Cluster; Evaporation; Secondary Electron; Photo-cathode; Electron Scattering; Energy Loss Function; Single Particle Imaging; X-ray Diffraction; Water; Ice; KI; CsI; Molekylär biofysik;

   Abstract : X-ray lasers bring us into a new world in photon science by delivering extraordinarily intense beams of x-rays in very short bursts that can be more than ten billion times brighter than pulses from other x-ray sources. These lasers find applications in sciences ranging from astrophysics to structural biology, and could allow us to obtain images of single macromolecules when these are injected into the x-ray beam. READ MORE

7. 5. Advanced Genomic Tools in Translational Research of Neurodevelopmental Disorders

   Author : Josefin Johansson; Maria Wilbe; Joris Vermeesch; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; neurodevelopmental disorders; translational research; advanced genomic technologies; Medicinsk genetik; Medical Genetics;

   Abstract : Neurodevelopmental disorders (NDDs) affect >4.7% of individuals world-wide. Most cases are expected to have an underlying genetic cause. However, only 36–40% of those affected get a genetic diagnosis through exome sequencing. READ MORE