Search for dissertations about: "single-molecule imaging"

Showing result 1 - 5 of 35 swedish dissertations containing the words single-molecule imaging.

  2. 1. Single Molecule Detection : Microfluidic Automation and Digital Quantification

    Author : Malte Kühnemund; Mats Nilsson; Ulf Landegren; Sten Linnarsson; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; single molecule; digital; rolling circle amplification; magnetic particle; padlock probe; microfluidics; resistive pulse sensing; lab on chip; mobile phone microscopy; enrichment; sequencing;

    Abstract : Much of recent progress in medical research and diagnostics has been enabled through the advances in molecular analysis technologies, which now permit the detection and analysis of single molecules with high sensitivity and specificity. Assay sensitivity is fundamentally limited by the efficiency of the detection method used for read-out. READ MORE

  4. 2. Probing Biomolecular Recognition at the Single Molecule Level

    Author : Anders Gunnarsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Abstract : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. READ MORE

  5. 3. Single-­molecule spectroscopy of π-­conjugated polymers

    Author : Oleg Mirzov; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescence lifetime; low temperature; spectral diffusion; blinking; MEH-PPV; Single-molecule spectroscopy; π-conjugated polymers; 2D polarization; Monte Carlo; energy transfer;

    Abstract : When applied to condensed-matter samples of large organic molecules, optical spectroscopy faces the problem of inhomogeneous broadening caused by the diversity of environments and conformations that sample molecules are subjected to. This severely limits the information that can be extracted from optical spectra. READ MORE

  6. 4. Förster Resonance Energy Transfer - from single molecule spectroscopy to imaging

    Author : Evangelos Sisamakis; Jerker Widengren; Paul R Selvin; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; FRET; FCS; single-molecule biophysics;

    Abstract : During the last fifteen years several methods have been developed for probing biomolecules (DNA, RNA, proteins) one at a time. Among these methods fluorescence spectroscopy and in particular its many implementations for monitoring Förster Resonance Energy Transfer (FRET), have attracted much interest. READ MORE

  7. 5. Polarization portraits of lightharvesting antennas: from single molecule spectroscopy to imaging

    Author : Rafael Camacho Dejay; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Energy transfer; Single molecule spectroscopy; Exciton; Fluorescence microscopy; Fluorescence polarization; Light-harvesting; Chromophore; Conjugated Polymer; Photosynthesis;

    Abstract : Multichromophoric systems are very important in photosynthesis and any device that uses solar energy for its operation. This is because multichromophoric light-harvesting antennas are responsible for the absorption of light and the efficient transfer of the absorbed energy toward distinct places where it is to be used or stored. READ MORE