Search for dissertations about: "single-molecule level"

Showing result 1 - 5 of 45 swedish dissertations containing the words single-molecule level.

  2. 1. Probing Biomolecular Recognition at the Single Molecule Level

    Author : Anders Gunnarsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; cell membrane; TIRFM; liposome; time-of-flight secondary ion mass spectrometry; membrane receptor; single molecule; TOF-SIMS; molecular recognition; DNA; barcode; total internal reflection; multiplexing;

    Abstract : Specific recognition between biomolecular partners contributes to a multitude of biochemical signaling within and between cells. The aim of this work has been to investigate the possibility to probe biomolecular recognition reactions at the smallest possible scale, the single molecule level. READ MORE

  4. 2. Overstretching short DNA – Single-molecule force spectroscopy studies

    Author : Niklas Bosaeus; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; DNA stretching; mechanical properties of DNA; single molecule; optical tweezers; DNA structure;

    Abstract : Structural deformation of DNA has a central role in many biological processes. It occurs, for example, during replication, transcription, and regulation of the activity of the genome. To understand these fundamental processes it is necessary to have a detailed knowledge of the mechanical properties of DNA. READ MORE

  5. 3. Single-molecule Detection in situ

    Author : Chatarina Larsson; Mats Nilsson; Ulf Landegren; Sanjay Tyagi; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Molecular medicine; Molekylär medicin; Molecular Medicine; molekylär medicin genetik och patologi ;

    Abstract : The human body contains a variety of different cell types that share a common genome, but differ in how they use the information encoded therein. Variation in molecular content exists even at the level of individual cells, and to provide deeper insight into complex cellular processes methods that permit analysis of each cell on its own are needed. READ MORE

  6. 4. From single-cell transcriptomics to single-molecule counting

    Author : Saiful Islam; Karolinska Institutet; Karolinska Institutet; []
    Keywords : ;

    Abstract : RNA-sequencing (RNA-seq) technology has been progressing so fast in the last few years and made it possible to perform transcriptome analysis at single-cell level that was even unimaginable a few years before. Nowadays, the importance of gene expression analysis at the single-cell level is increasingly appreciated for the study of complex heterogeneous tissue. READ MORE

  7. 5. Light polarization in single molecule spectroscopy: From visualization of structural anisotropy and energy transfer to super-resolution imaging with orientational contrast

    Author : Daniel Thomsson; Kemisk fysik; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Single-molecule spectroscopy; π-conjugated polymers; blinking; fluorescence brightness; low temperature; polarization anisotropy; 2D polarization; energy transfer; super resolution; Fourier transform;

    Abstract : Π-conjugated polymers are an important class of materials which can be used in e.g. photoelectric (solar cells) or electroluminescent (displays) devices. These materials are implemented in a number of applications these days since a lot is already known about them. READ MORE