Search for dissertations about: "site specific labeling"

Showing result 1 - 5 of 29 swedish dissertations containing the words site specific labeling.

  1. 1. Development of a covalent site-specific antibody labeling strategy by the use of photoactivable Z domains

    Author : Anna Konrad; Sophia Hober; Hjalmar Brismar; Patrik Samuelsson; KTH; []
    Keywords : antibody labeling; site-specific; covalent; SPPS; Z domain; BPA; SRA - Molekylär biovetenskap; SRA - Molecular Bioscience;

    Abstract : The joining of two molecular functions or the strategy of adding functions to proteins has been tremendously important for the development of proteins as tools in research and clinic. Depending on the intended application, there are a wide variety of functions that can be added to a proteins. READ MORE

  2. 2. Site-specific labeling of affinity molecules for in vitro and in vivo studies

    Author : Anna Perols; Amelie Eriksson Karlström; Christian Hackenberger; KTH; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Affibody molecules; molecular imaging; site-specific labeling; solid phase peptide synthesis; IgG-binding domains; photoconjugation.; Bioteknologi; Biotechnology;

    Abstract : The thesis is focused on site-specific labeling of affinity molecules for different applications where two types of binding proteins, Affibody molecules and antibodies, have been used. For the purpose of improving the properties of Affibody molecules for in vivo imaging, novel bi-functional chelators for radiolabeling using the radionuclide 111In were evaluated. READ MORE

  3. 3. Labeling, tagging and charging - Long standing issues in bioenergetics addressed with novel techniques

    Author : Tobias Gustavsson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; WrbA; site specific labeling; fusion proteins; spectro electrochemistry; succinate quinone reductase; NADH quinone oxidoredictase; cytochromes; Respiratory chain;

    Abstract : Understanding the molecular details of bioenergetic processes is fundamentally important. Defects in the mitochondrial energy metabolism can lead to untimely apoptosis and thus cause serious degenerative human diseases. READ MORE

  4. 4. Modulating Sirtuin Activity - Design, Synthesis and Evaluation of Sirtuin 2 Inhibitors

    Author : Tina Seifert; Göteborgs universitet; Göteborgs universitet; Gothenburg University; []
    Keywords : Sirtuin; SIRT2; Inhibitors; Chroman-4-ones; Chromones; Benzothiadiazine-1; 1-dioxides; Saccharin; Scaffold; Structure-activity relationship; Antiproliferative properties; Binding site; Homology modeling; Photoaffinity labeling; Diazirine; Mass spectrometry;

    Abstract : Sirtuins (SIRTs) are NAD+-dependent lysine deacetylating enzymes targeting histones and a multitude of non-histone proteins. The SIRTs have been related to important cellular processes such as gene expression, cell proliferation, apoptosis and metabolism. They are proposed to be involved in the pathogenesis of e.g. READ MORE

  5. 5. Fluorescent Probes and Protein Misfolding: Methods and Applications

    Author : Irem Nasir; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein misfolding; fluorophores; filter-trap; site-specific fluorophore labeling; amyloid-beta; microscopic rate constants; solvatochromic probes; alpha-synuclein; lipid vesicles; nanoparticles; protein adsorption;

    Abstract : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. READ MORE