Search for dissertations about: "site specific labeling"
Showing result 21 - 25 of 35 swedish dissertations containing the words site specific labeling.
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21. Biophysical Studies of Protein Folding, Aggregation and Interactions with Molecular Chaperones
Abstract : The information for a protein to fold correctly into a well defined three-dimensional functional structure is embedded in the primary sequence. Much effort has been devoted to characterize the nature of the unfolded state, since this state represents the starting point of the folding reaction. READ MORE
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22. Synaptic organization of nociceptive relay nuclei in the cat and primate thalamus
Abstract : The fine structure of two thalamic nociceptive relay nuclei, the nucleus submedius (Sm) in the cat, and the posterior region in the owl monkey, was investigated using anatomical tract tracing techniques, immunohistochemical staining for neuroactive substances, and threedimensional reconstructions of synaptic circuitry. Ascending lamina I axons, labeled by anterograde transport of Phaseolus vulgaris leucoagglutinin, gave rise to two different types of termination in Sm: large, compact clusters of numerous boutons were predominant in the dense focus of the terminal field after a tracer injection at the site of nociceptive-specific neurons in lamina I, and boutons-of-passage type terminations were conspicuous in the periphery of a lamina I terminal field in Sm and predominant following a tracer injection at the site of thermoreceptive-specific neurons in lamina I. READ MORE
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23. Development of phosphorus-containing metallo-β-lactamase inhibitors : Synthesis and binding studies by solution NMR and molecular docking
Abstract : The rapidly growing bacterial resistance development is turning into one of the main challenges of the 21st century. Our antibiotics are becoming ineffective for the treatment of bacterial infections, and without successful action, simple infections, such as pneumonia or Septicemia, will soon carry a highly probable mortal prognosis. READ MORE
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24. RNA systems for NMR studies in vitro and in vivo
Abstract : NMR spectroscopy is an excellent tool to study the structure-function relationship of RNA. Such measurements are usually performed in vitro, which requires large amounts of isotope- labeled sample in high purity and can give access to individual atoms, structure of the molecule and conformational dynamics. READ MORE
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25. Glutaredoxin 3 from Escherichia coli : NMR structure analysis and structural aspects of the enzymatic mechanism
Abstract : The structure of Escherichia coli glutaredoxin 3 (Grx3) was investigated using NMR spectroscopy in combination with isotope labeling. Nearly complete sequence specific assignment were obtained for the reduced Grx3 using a 15N enriched protein sample. READ MORE
