Search for dissertations about: "site-specific fluorophore labeling"
Found 2 swedish dissertations containing the words site-specific fluorophore labeling.
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1. Fluorescent Probes and Protein Misfolding: Methods and Applications
Abstract : Protein misfolding is a frequently occurring event in living cells and without the quality control, the consequences are disasterous. In this thesis, we studied protein misfolding in vitro by employing a variety of fluorophores, to either detect the highly ordered aggregates of intrinsically disordered proteins/peptides or slight misfolding of a natively folded protein. READ MORE
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2. A Novel Route for Construction of Multipurpose Receptors through Chemical Modification of Glutathione Transferases
Abstract : This thesis describes how the human Alpha class glutathione transferase (GST) A1-1 can be reprogrammed either to function as a multipurpose biosensor for detection of small molecule analytes, or as a handle providing for more efficient protein purification.A novel, user-friendly, and efficient method for site-specific introduction of functional groups into the active site of hGST A1-1 is the platform for these achievements. READ MORE