Search for dissertations about: "solid-phase refolding"

Found 2 swedish dissertations containing the words solid-phase refolding.

  2. 1. Engineering of the Ultra-stable Cystine Knot Framework of Microproteins : Design, Chemical Synthesis and Structural Studies

    Author : Teshome Leta Aboye; Ulf Göransson; Julio A. Camarero; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cyclotides; cystine knot; protein engineering; NMR; solid phase; disulfide rich; ultra-stable; microprotein; PHARMACY; FARMACI; Medicinal Chemistry; Läkemedelskemi;

    Abstract : Ultra-stable cystine knotted microproteins, in which two disulfides and their connecting backbones form a circle that is penetrated by the third disulfide bonds, have attracted high interest due to their resistance to degradation in vitro and potential for the development of peptide drugs. This thesis gives new insights into engineering of that framework of microproteins, including approaches to their chemical synthesis, backbone engineering, structural and biological evaluations. READ MORE

  4. 2. Strategies for facilitated protein recovery after recombinant production in Escherichia coli

    Author : My Hedhammar; Sophia Hober; Alois Jungbauer; KTH; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; ion-exchange chromatography; protein A; Z; Zbasic; Zacid; fusion protein; proteolytic cleavage; immobilised protease; flow cytometry; inclusion bodies; solid-phase refolding; Molecular biology; Molekylärbiologi;

    Abstract : The successful genomic era has resulted in a great demand for efficient production and purification of proteins. The main objective of the work described in this thesis was to develop methods to facilitate recovery of target proteins after recombinant production in Escherichia coli. READ MORE