Search for dissertations about: "solution hybridization"

Showing result 1 - 5 of 28 swedish dissertations containing the words solution hybridization.

  1. 1. Concatemerization of Synthetic Oligonucleotides

    Author : Lu Sun; [2014]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; gel electrophoresis; DNA-binding; QCM-D; DNA concatemer; hybridization; DNA formation; self-assembly; self-assemble;

    Abstract : DNA nanotechnology has become an important research field because of its advantages in high predictability and accuracy of base-paring recognition. For example, the DNA concatemer, one of the simplest DNA constructs in shape, has been used to enhance signals in biosensing. READ MORE

  2. 2. The Light-up Probe

    Author : Nicke Svanvik; [2000]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; asymmetric cyanine dyes; light-up probes; PNA; real-time PCR; LightUp Probe; fluorescent hybridization probes; homogeneous hybridization assays;

    Abstract : The work presented in this thesis concerns the development of light-up probes and their potential applications in PCR based molecular diagnostics. The light-up probe is a nucleic acid analog (here PNA) oligomer linked to an asymmetric cyanine dye (here thiazole orange) and it combines the excellent hybridization properties of PNA with the large fluorescence enhancement of the dye upon binding to DNA. READ MORE

  3. 3. Genotyping RNA and DNA using padlock probes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Dan-Oscar Antson; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Genetics; Padlock probe; enzymatic synthesis; PCR; in situ hybridization; RNA-templated DNA ligation; Genetik; MEDICINE Dermatology and venerology; clinical genetics; internal medicine Clinical genetics; MEDICIN Dermatologi och venerologi; klinisk genetik; invärtesmedicin Klinisk genetik; Medical Genetics; medicinsk genetik;

    Abstract : Novel techniques are needed to investigate the genetic variation revealed in the first draft of the human genome sequence. Padlock probes are recently developed reagents, suitable for detecting single-nucleotide variations of DNA and RNA in situ or in solution. READ MORE

  4. 4. Molecular pharmacology of amiodarone

    University dissertation from Stockholm : Karolinska Institutet, Department of Medicine

    Author : Viktor Drvota; Karolinska Institutet.; Karolinska Institutet.; [1997]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Amiodarone; thyroid hormone receptors; B-adrenergic receptors; AT-1 cardiomyocytes; Western blot; solution hybridization; Fura-2; Langendorff heart; mouse; guinea pig;

    Abstract : MOLECULAR PHARMACOLOGY OF AMIODARONE Amiodarone (Am) is a potent class III antiarrhythmic drug. Its use is limited due to serious side effects and it was therefore used only when other therapeutic interventions proved to be ineffective. READ MORE

  5. 5. Engineering of the Ultra-stable Cystine Knot Framework of Microproteins Design, Chemical Synthesis and Structural Studies

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Teshome Leta Aboye; Ulf Göransson; Julio A. Camarero; [2011]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; cyclotides; cystine knot; protein engineering; NMR; solid phase; disulfide rich; ultra-stable; microprotein; PHARMACY; FARMACI; Medicinal Chemistry; Läkemedelskemi;

    Abstract : Ultra-stable cystine knotted microproteins, in which two disulfides and their connecting backbones form a circle that is penetrated by the third disulfide bonds, have attracted high interest due to their resistance to degradation invitro and potential for the development of peptide drugs. This thesis gives new insights into engineering of that framework of microproteins, including approaches to their chemical synthesis, backbone engineering, structural and biological evaluations. READ MORE