Search for dissertations about: "spin-labels"

Showing result 1 - 5 of 9 swedish dissertations containing the word spin-labels.

  2. 1. Theoretical studies of EPR parameters of spin-labels incomplex environments

    Author : Bogdan Frecus; Hans Ågren; Chiara Cappelli; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; EPR; DFT; spin-labels; QM MM; Breit-Pauli Hamiltonian;

    Abstract : This thesis encloses quantum chemical calculations performed in the framework of density functional response theory for evaluating electron paramagnetic resonance (EPR) spin Hamiltonian parameters of various spin-labels in different environments. These parameters are the well known electronic g-tensor and the nitrogen hyperfine coupling constants, which are extensively explored in this work for various systems. READ MORE

  4. 2. Lipase-Lipid Interactions Studied by Site-Directed Labeling and Biological Spectroscopy

    Author : Eva Hedin; Karl Hult; Marek Brzozowski; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; thermomyces lanuginous lipase; site-directed labeling; lipase-lipid interactions; Biochemistry; Biokemi;

    Abstract : This thesis describes the study of lipase–lipid interactions of the triglyceride lipase Thermomyces lanuginosus lipase, TLL, by use of site-directed chemical labeling and biological spectroscopy. Several single-cysteine variants of TLL were produced, and labeled with probes containing either an affinity-, a fluorescence- or a spin-label functionality. READ MORE

  5. 3. Structure Analysis of a Transient Protein Complex - NMR, X-ray crystallographic and Mass Spectrometric Studies of the Soluble Domains of the Proton-Pump Transhydrogenase from E. coli

    Author : Tomas Johansson; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; nuclear magnetic resonance; proton-translocating transhydrogenase; structure; interactions; x-ray crystallography;

    Abstract : Interactions between macromolecules in the cell are fundamental to life. However, many interactions are of a transient nature and are as such difficult to study at the atomic level using existing techniques. READ MORE

  6. 4. Molecular dynamics in membrane systems : an ESR spin label study

    Author : Magdalena Ehrström; Stockholms universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The ESR spin labeling technique has been used to investigate order and motion in medel lipid systems and in biological membranes under various experimental conditions.The effects of CaCl2, MgCl2, LaCl3 and some alkali halides on B. READ MORE

  7. 5. Studies of Local Interactions between and within Proteins using Site-Directed Labeling Techniques

    Author : Rikard Owenius; Lawrence J. Berliner; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : Proteins are essential participants in virtually all cellular processes. The key to the understanding of the function of a certain protein is a detailed knowledge about its atomic three-dimensional structure. READ MORE