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Showing result 1 - 5 of 9 swedish dissertations matching the above criteria.
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1. Vectorization of oligonucleotides with cell-penetrating peptides : Characterization of uptake mechanisms and cytotoxicity
Abstract : The hydrophobic plasma membrane constitutes an indispensable barrier for cells in living animals. Albeit being pivotal for the maintenance of cells, the inability to cross the plasma membrane is still one of the major obstacles to overcome in order to progress current drug development. READ MORE
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2. Design and application of RNA therapeutics for splice site mutations
Abstract : Precursor messenger RNA splicing is one of the most fundamental and complex mechanisms in eukaryotes. Over 90% of the human genes undergo alternative splicing, which is essential for the regulation of gene expression. A dynamic RNA-protein complex called the spliceosome catalyzes splicing. READ MORE
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3. Cell-penetrating peptides in protein mimicry and oligonucleotide delivery : Applications and mechanisms
Abstract : The plasma membrane functions as a barrier, restricting entry of hydrophilic pharmaceutical agents. Cell-penetrating peptides (CPPs) are capable of transporting bioactive cargos into the cell and have consequently been extensively investigated for their mechanism of entry and capability to deliver various cargos spanning from peptides to plasmids. READ MORE
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4. Delivery of gene-regulating agents : internalization mechanisms and novel vectors
Abstract : The sequencing of the human genome was expected to generate a veritable explosion of therapeutics for regulation of gene function, either through modulation of gene expression or through the replacement of defect genes. However, nucleic acid-based pharmacological agents suffer from issues of low bioavailability and unfavorable pharmacokinetics, wherefore these prospects have not yet been realized. READ MORE
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5. Nucleic acid delivery : reports from the search of the magic bullet
Abstract : Gene therapy is regarded as one of the most promising therapeutic approaches, since it has the potential to treat disorders by correcting malformations at the nucleic acids level rather than proteins, as opposed to conventional medicine. However, for non-viral gene therapy to successfully fulfill the requirements associated to the “magic bullet” has proven difficult. READ MORE