Search for dissertations about: "split-GFP"

Showing result 1 - 5 of 6 swedish dissertations containing the word split-GFP.

  1. 1. Cell line and protein engineering tools for production and characterization of biologics

    Author : Anna-Luisa Volk; Johan Rockberg; Mathias Uhlen; Dickson Alan; KTH; []
    Keywords : ENGINEERING AND TECHNOLOGY; TEKNIK OCH TEKNOLOGIER; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Pharmaceutical proteins; precision medicine; antibody engineering; epitope mapping; cell line development; split-GFP; Biotechnology; Bioteknologi;

    Abstract : Our increasing understanding of disease mechanisms coupled with technological advances has facilitated the generation of pharmaceutical proteins, which are able to address yet unmet medical needs. Diseases that were fatal in the past can now be treated with novel biological medications improving and prolonging life for many patients. READ MORE

  2. 2. To Fold or To Fibrillate? Serendipity in Stability Studies

    Author : Olga Szczepankiewicz; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Protein stability; protein reconstitution; protein aggregation; Monellin; PGB1; Split-GFP; Amyloid beta;

    Abstract : Proteins are complex structures and years of research have been spent on attempts to understand their complexity. The non-covalent interactions involved in protein folding are: hydrophobic effect, electrostatic interactions, van der Waals interactions and hydrogen bonding. READ MORE

  3. 3. Methods for cell line and protein engineering

    Author : Magnus Lundqvist; Johan Rockberg; Mathias Uhlén; Diethard Mattanovich; KTH; []
    Keywords : MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Cell line development; therapeutic proteins; protein engineering; molecular cloning; mutagenesis; split-GFP; Biotechnology; Bioteknologi;

    Abstract : Therapeutic proteins are becoming increasingly important. They are desirable, as they typically possess low adverse effects and higher specificity compared to the traditional, small molecule drugs. But they are also more complex and involve different intricate and expensive development and production processes. READ MORE

  4. 4. Perplexing Protein Puzzles

    Author : Stina Lindman; Biofysikalisk kemi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; calbindin D9k; split-GFP; protein G B1; pKa-values; protein reconstitution; Electrostatic interactions; protein stability; protein denaturation; heteronuclear NMR; pH titrations;

    Abstract : Protein structure and stability are inherent in the amino acid sequence and governed by non-covalent interactions. The cooperation between forces is, however, perplexing and not well understood. In order to elucidate and predict protein folding and stability, detailed studies of non-covalent interactions are required. READ MORE

  5. 5. Engineering membrane proteins for production and topology

    Author : Stephen Toddo; Daniel Daley; Roslyn Bill; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Membrane protein; Over-expression; Protein production; Codon optimization; Escherichia coli; AraH; NarK; mRNA secondary structure; coding sequence; ribosome binding site; RBS; topology; split-GFP; biokemi; Biochemistry;

    Abstract : The genomes of diverse organisms are predicted to contain 20 – 30% membrane protein encoding genes and more than half of all therapeutics target membrane proteins. However, only 2% of crystal structures deposited in the protein data bank represent integral membrane proteins. READ MORE