Search for dissertations about: "stable duplex"

Showing result 1 - 5 of 17 swedish dissertations containing the words stable duplex.

2. 1. Conformationally Constrained Nucleosides : Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides

   Author : Oommen P. Varghese; Lars Engman; Jacek Stawinski; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; chemically modified oligonucleotides; azetidine; aza-ENA-T; cyanomethylene locked; free energy of activation; diaxial interactions; chair conformation; stable duplex; human serum; snake venom phosphodiesterase; antisense agents; RNase H; Organisk kemi; Chemistry; Kemi;

   Abstract : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. READ MORE

4. 2. The fracture mechanisms in duplex stainless steels at sub-zero temperatures

   Author : Johan Pilhagen; Pavel Korzhavy; Rolf Sandström; Kim Wallin; KTH; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Duplex stainless steel; Weldments; Delamination; Fracture toughness; Impact toughness; Cleavage fracture; Nickel;

   Abstract : The aim of the thesis was to study the susceptibility for brittle failures and the fracture process of duplex stainless steels at sub-zero temperatures (°C). In the first part of the thesis plates of hot-rolled duplex stainless steel with various thicknesses were used to study the influence of delamination (also known as splits) on the fracture toughness. READ MORE

5. 3. NMR structral studies on ruthenium complexes binding to DNA

   Author : Lisha Wu; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NMR; DNA binding; Ru-complex dimer; CD; fluorescence; molecular dynamics simulation;

   Abstract : The Ru-complex Λ,Λ-[bidppz(bipy)4Ru2]4+ (Λ,Λ-B) exhibits multiple modes of interactions with the palindromic DNA dodecamer [d(CGCGAATTCGCG)]2, however excluding any type of intercalation as shown by both optical spectroscopy and NMR. NMR observation of Λ,Λ-B titration into a DNA solution results in both a transient binding, with observation of fast exchange for protons located near the ends of the DNA duplex, and a more stable interaction in the DNA minor groove indicated by a slow exchange mechanism and binding sites for G2, G4 and A5 of the same DNA strand. READ MORE

6. 4. Creep Behaviour of Titanium Aluminides and its Relation to Phase Distributions and Dislocation Structures

   Author : Dennis Lundström; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; gamma-TiAl; twinning; fatigue; dislocations; microstructure; thermal transport properties; creep;

   Abstract : The need for more efficient gas turbines with lowered fuel consumption and thereby lowered emission has recently lead to increased interest in low-density, .gamma.-based TiAl intermetallic alloys as a replacement of e.g. READ MORE

7. 5. Structural Adaptation of DNA to Small Ligands and Recombination Proteins

   Author : Hye-Kyung Kim; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; triplex DNA; groove binders; intercalators; SANS; DNA; optical spectroscopy; recombination proteins;

   Abstract : In order to contribute to the understanding of mechanisms of recognition in nucleic acid systems, the interaction of DNA with a variety of ligands, representing different cases of adaptation of the double-helix structure, was studied using flow linear dichroism (LD), circular dichroism (CD), fluorescence and absorption spectroscopic techniques. A chiral hydrophobic metal complex, [Ru(dpphen)3]2+ was examined, since this had been reported to be the most efficient molecule to discriminate DNA handedness. READ MORE