Search for dissertations about: "stable duplex"

Showing result 1 - 5 of 12 swedish dissertations containing the words stable duplex.

  1. 1. Conformationally Constrained Nucleosides Design, Synthesis, and Biochemical Evaluation of Modified Antisense Oligonucleotides

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Oommen P. Varghese; Uppsala universitet.; [2007]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Organic chemistry; chemically modified oligonucleotides; azetidine; aza-ENA-T; cyanomethylene locked; free energy of activation; diaxial interactions; chair conformation; stable duplex; human serum; snake venom phosphodiesterase; antisense agents; RNase H; Organisk kemi; NATURAL SCIENCES Chemistry; NATURVETENSKAP Kemi;

    Abstract : This thesis is concerned with synthesis, structure and biochemical analysis of chemically modified oligonucleotides with potential therapeutic applications. The three types of chemical modifications described here are: (a) A North-East locked 1',2'-azetidine nucleoside (b) A North locked 2',4'-cyanomethylene bridged nucleoside and (c) A 2',4'-aza-ENA-T nucleoside. READ MORE

  2. 2. The fracture mechanisms in duplex stainless steels at sub-zero temperatures

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Johan Pilhagen; KTH.; [2013]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Duplex stainless steel; Weldments; Delamination; Fracture toughness; Impact toughness; Cleavage fracture; Nickel;

    Abstract : The aim of the thesis was to study the susceptibility for brittle failures and the fracture process of duplex stainless steels at sub-zero temperatures (°C). In the first part of the thesis plates of hot-rolled duplex stainless steel with various thicknesses were used to study the influence of delamination (also known as splits) on the fracture toughness. READ MORE

  3. 3. NMR structral studies on ruthenium complexes binding to DNA

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Lisha Wu; [2011]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NMR; DNA binding; Ru-complex dimer; CD; fluorescence; molecular dynamics simulation;

    Abstract : The Ru-complex Λ,Λ-[bidppz(bipy)4Ru2]4+ (Λ,Λ-B) exhibits multiple modes of interactions with the palindromic DNA dodecamer [d(CGCGAATTCGCG)]2, however excluding any type of intercalation as shown by both optical spectroscopy and NMR. NMR observation of Λ,Λ-B titration into a DNA solution results in both a transient binding, with observation of fast exchange for protons located near the ends of the DNA duplex, and a more stable interaction in the DNA minor groove indicated by a slow exchange mechanism and binding sites for G2, G4 and A5 of the same DNA strand. READ MORE

  4. 4. Cationic Interactions with Nucleic Acids With Focus on Anticancer Active Platinum(II) Complexes

    University dissertation from Department of Chemistry, Lund University

    Author : Åse Sykfont Snygg; Lunds universitet.; Lund University.; [2008]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; in-line cleavage; Cisplatin; oxaliplatin; circular dichroism; DENSPM; polyamines; siRNA; pseudoknot; RNA; thermal melting; anticancer active Pt II complex; DNA;

    Abstract : It is well documented that the antitumour activity of the Pt(II)-complex cisplatin, and similar anticancer active complexes, is a result of metalation of DNA, and thereby inhibition of cell proliferation. Since nucleic acids are polyanions at physiological pH, the rate of adduct formation with positively charged complex, as the hydrolyzed Pt(II) complexes studied in this work, is influenced by the ionic environment surrounding the polymer. READ MORE

  5. 5. Activation of Herpes simplex virus type 1 origin of DNA replication

    University dissertation from Göteborg

    Author : Alireza Aslani; Göteborgs universitet.; Gothenburg University.; [2002]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; DNA replication; Herpes simplex virus; helicase; UL9; OBP; ICP8; ATP; oriS; hairpin;

    Abstract : Herpes simplex virus type 1 has been used to study the molecular mechanism of DNA replication. Initiation of DNA replication takes place at specific regions called origins of replication. The HSV-1 genome contains three origins of replication, one copy of oriL and two copies of oriS. READ MORE