Advanced search
Showing result 1 - 5 of 26 swedish dissertations matching the above criteria.
-
1. Bacterial display systems for engineering of affinity proteins
Abstract : Directed evolution is a powerful method for engineering of specific affinity proteins such as antibodies and alternative scaffold proteins. For selections from combinatorial protein libraries, robust and high-throughput selection platforms are needed. READ MORE
-
2. Staphylococcal surface display for protein engineering and characterization
Abstract : Even though our understanding of mechanisms such as protein folding and molecular recognition is relatively poor, antibodies and alternative affinity proteins with entirely novel functions are today generated in a routine manner. The reason for this success is an engineering approach generally known as directed evolution. READ MORE
-
3. Staphylococcal surface display in directed evolution
Abstract : Engineered affinity proteins have together with naturally derived antibodies becomeindispensable tools in many areas of life-science and with the increasing number ofapplications, the need for high-throughput methods for generation of such different affinityproteins is evident. Today, combinatorial protein engineering is the most successful strategy toisolate novel non-immunoglobulin affinity proteins. READ MORE
-
4. Utilizing Solid Phase Cloning, Surface Display And Epitope Information for Antibody Generation and Characterization
Abstract : Antibodies have become indispensable tools in diagnostics, research and as therapeutics. There are several strategies to generate monoclonal antibodies (mAbs) in order to avoid the drawbacks of polyclonal antibodies (pAbs) for therapeutic use. READ MORE
-
5. Engineering strategies for ABD-derived affinity proteins for therapeutic and diagnostic applications
Abstract : Small stable protein domains are attractive scaffolds for engineering affinity proteins due to their high tolerance to mutagenesis without loosing structural integrity. The albuminbinding domain is a 5 kDa three-helix bundle derived from the bacterial receptor Protein G with low-nanomolar affinity to albumin. READ MORE