Search for dissertations about: "structural variant"

Showing result 1 - 5 of 104 swedish dissertations containing the words structural variant.

2. 1. Combined structural methods to characterize an oligomeric small heat shock protein Hsp21

   Author : Gudrun Rutsdottir; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Small heat shock proteins; chaperones; crosslinking mass spectrometry; Stable isotope labeling; quantification; small angle X-ray scattering; cryo-electron microscopy; combined structural methods; plant stress;

   Abstract : Small heat shock proteins (sHsps) are found in all organisms and evolved early in the history of life, prior to the divergence of the three domains of life (Archaea, Bacteria, and Eukarya). Their malfunction is connected to several different diseases in humans, such as Alzheimer’s and Parkinson’s disease. READ MORE

4. 2. Structure Function Relationships in Pyrimidine Degrading and Biocatalytic Enzymes, and Their Implications for Cancer Therapy and Green Chemistry

   Author : Dirk Maurer; Doreen Dobritzsch; Mikael Widersten; Rikkert Wierenga; Uppsala universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; β-Ureidopropionase; Dihydropyrimidine Dehydrogenase; Alcohol Dehydrogenase; Pyrimidine catabolism; 5-Fluorouracil; CASTing; X-ray Crystallography;

   Abstract : This thesis includes the work of two separate projects, studies on pyrimidine degrading enzymes and studies on in vitro evolved enzymes. The common denominator of both projects was the use of structural information to explain functional effects, observed in the studied biocatalysts. READ MORE

5. 3. Thiopurine S-methyltransferase - characterization of variants and ligand binding

   Author : Annica Blissing; Lars-Göran Mårtensson; Tomas Nyman; Linköpings universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Thiopurine S-methyltransferase TPMT ; protein stability; enzyme kinetics; ligand binding; isothermal titration calorimetry ITC ; 8-anilinonaphthalene-1-sulfonic acid ANS ;

   Abstract : Thiopurine S-methyltransferase (TPMT) belongs to the Class I S-adenosylmethionine-dependent methyltransferase (SAM-MT) super family of structurally related proteins. Common to the members of this large protein family is the catalysis of methylation reactions using S-adenosylmethionine (SAM) as a methyl group donor, although SAM-MTs act on a wide range of different substrates and carry out numerous biologically important functions. READ MORE

6. 4. High (valent) on O2 : Ribonucleotide Reductase and Methane Monooxygenase

   Author : Juliane John; Martin Högbom; Pål Stenmark; Peter Moody; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Ribonucleotide Reductase; Methane Monooxygenase; biokemi; Biochemistry;

   Abstract : Macromolecular X-ray crystallography (MX) is a powerful method to investigate protein structures. However, proteins with redox-active centres and radicals are very susceptible to photoreduction. READ MORE

7. 5. Exploring Unorthodox Aquaporins : Characterization of NbXIPs & AtNIPs

   Author : Henry Ampah-Korsah; Biokemi och Strukturbiologi; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Aquaporins; XIPs; NIPs; Phosphorylation; Pichia pastoris; Overexpression; boric acid; membrane proteins;

   Abstract : AbstractAquaporins are membrane integral proteins that facilitate the transport of waterand/or other small neutral molecules across biological membranes in cells in allforms of life. Among the subfamilies of aquaporins in higher plants, the X IntrinsicProteins (XIPs) are the most recently discovered subfamily and the leastcharacterized. READ MORE