Search for dissertations about: "subcellular location"

Showing result 1 - 5 of 19 swedish dissertations containing the words subcellular location.

2. 1. Antibody-based subcellular localization of the human proteome

   Author : Marie Skogs; Emma Lundberg; Hans Blom; Cecilia Williams; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Human proteome; Subcellular localization; Organelles; Immunofluorescence; Fixation; Permeabilization; Antibody validation; Bioteknologi; Biotechnology;

   Abstract : This thesis describes the use of antibodies and immunofluorescence for subcellular localization of proteins. The key objective is the creation of an open-source atlas with information on the subcellular location of every human protein. READ MORE

4. 2. Atypical Solute Carriers : Identification, evolutionary conservation, structure and histology of novel membrane-bound transporters

   Author : Emelie Perland; Robert Fredriksson; Helgi Schiöth; Åsa Mackenzie; Sonchita Bagchi; Carsten Uhd Nielsen; Uppsala universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Major facilitator superfamily; solute carrier; transporter; protein expression; mRNA expression; phylogenetic clustering; orthologues; co-expression; subcellular location; nutrition.; Neuroscience; Neurovetenskap;

   Abstract : Solute carriers (SLCs) constitute the largest family of membrane-bound transporter proteins in humans, and they convey transport of nutrients, ions, drugs and waste over cellular membranes via facilitative diffusion, co-transport or exchange. Several SLCs are associated with diseases and their location in membranes and specific substrate transport makes them excellent as drug targets. READ MORE

5. 3. Predicting the route: from protein sequence to sorting in eukaryotic cell

   Author : Marco Salvatore; Arne Elofsson; Norman Davey; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; eukaryotic cell; sorting signals; subcellular localisation; machine learning; biological sequence analysis; bioinformatics; Biochemistry towards Bioinformatics; biokemi med inriktning mot bioinformatik;

   Abstract : Proteins need to be localised in the correct compartment of a eukaryotic cell to function correctly. Therefore, a protein needs to be transported to the right location. Specific signals present in the protein sequence direct proteins to different subcellular localisations. READ MORE

6. 4. Mapping the human proteome using bioinformatic methods

   Author : Linn Fagerberg; Mathias Uhlén; Alvis Brazma; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; proteome; transcriptome; bioinformatics; membrane protein prediction; subcellular localization; protein expression level; cell line; immunohistochemistry; immunofluorescence; Bioinformatics; Bioinformatik; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : The fundamental goal of proteomics is to gain an understanding of the expression and function of the proteome on the level of individual proteins, on the level of defined cell types and on the level of the entire organism. In this thesis, the human proteome is explored using membrane protein topology prediction methods to define the human membrane proteome and by global protein expression profiling, which relies on a complex study of the location and expression levels of proteins in tissues and cells. READ MORE

7. 5. Finding order in chaos : Dissecting single-cell heterogeneity in space and time

   Author : Christian Gnann; Emma Lundberg; Manuel D Leonetti; Lucas Pelkmans; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; non-genetic single-cell heterogeneity; cell cycle; metabolism; imaging-based subcellular proteomics; phosphoproteomics; icke-genetisk single-cell heterogenitet; cellcykel; metabolism; bildbaserad subcellulär proteomik; fosfoproteomik; Biotechnology; Bioteknologi;

   Abstract : The cell is the smallest unit of life and contains DNA, RNA, proteins and a variety of other macromolecules. In recent years, technological advances in the field of single cell biology have revealed a staggering amount of phenotypic heterogeneity between cells in a population, which were previously considered homogenous. READ MORE