Search for dissertations about: "substrate specificity switch"

Showing result 1 - 5 of 7 swedish dissertations containing the words substrate specificity switch.

  1. 1. Rational engineering of esterases for improved amidase specificity in amide synthesis and hydrolysis

    University dissertation from Stockholm : KTH Royal Institute of Technology

    Author : Peter Hendil-Forssell; Mats Martinelle; Romas J. Kazlauskas; [2016]
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; Amidase; Biocatalysis; Enzyme; Esterase; Enzyme engineering; Lipase; Substrate specificity; Biotechnology; Bioteknologi;

    Abstract : Biocatalysis is an ever evolving field that uses enzymes or microorganisms for chemical synthesis. By utilizing enzymes that generally have evolved for specific reactions under mild conditions and temperatures, biocatalysis can be a more environmentally friendly option compared to traditional chemistry. READ MORE

  2. 2. Meticulous control of the T3SS of Yersinia is essential for full virulence

    University dissertation from Umeå : Institutionen för molekylärbiologi, Teknisk-naturvetenskaplig fakultet, Umeå universitet

    Author : Ann-Catrin Björnfot; Hans Wolf-Watz; Kelly T. Hughes; [2011]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Yersinia; type III secretion; YscU; substrate specificity switch; heat shock proteins; VagH; methyltransferase; virulence; NATURAL SCIENCES Chemistry Biochemistry Molecular biology; NATURVETENSKAP Kemi Biokemi Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : The type III secretion system (T3SS) of pathogenic Yersinia pseudotuberculosis is involved in virulence. The syringe-like secretion system spans both bacterial membranes and is responsible for the ability of Yersinia to transfer toxic proteins (Yop proteins) into the eukaryotic target cell. READ MORE

  3. 3. The Structure-Function Relationship and Evolution of Deoxyribonucleoside Kinases

    University dissertation from Department of Biology, Lund University

    Author : Zeeshan Mutahir; [2013]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES;

    Abstract : The synthesis of deoxyribonucleoside triphosphates (dNTPs), building blocks of DNA, can be achieved by two different pathways in the cell. One is called de novo synthesis and the other is known as salvage pathway. The de novo pathway involves synthesis of dNTPs by utilizing basic molecules like sugars, amino acids, CO2, NH3, etc. READ MORE

  4. 4. Structure and function of MMP-2 and its inhibitor TIMP-2

    University dissertation from Stockholm : Karolinska Institutet, Department of Medical Biochemistry and Biophysics

    Author : Ari Tuuttila; Karolinska Institutet.; Karolinska Institutet.; [2000]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Metalloproteinases; MMP-2; TIMP-2; extracellular matrix; crystallography; protein structure;

    Abstract : Animal cells secrete various enzymes capable of degrading the extracellular matrix. One of the most studied groups of these are the matrix metalloproteinases (MMPs). This group of zinc containing endopeptidases consists at present of 26 members and this number is still increasing. READ MORE

  5. 5. Structural Studies of Echinococcus granulosus Fatty-acid-binding Protein 1 and Human Semicarbazide-sensitive Amine Oxidase

    University dissertation from Stockholm : Karolinska Institutet, Department of Medical Biochemistry and Biophysics

    Author : Emma Jakobsson; Gerard Kleywegt; Andrea Mattevi; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Cell and molecular biology; semicarbazide-sensitive amine oxidase; vascular adhesion protein-1; copper-containing amine oxidase; fatty-acid-binding protein; Echinococcus granulosus; crystal structure; Cell- och molekylärbiologi; NATURAL SCIENCES Biology Cell and molecular biology; NATURVETENSKAP Biologi Cell- och molekylärbiologi;

    Abstract : The parasite Echinococcus granulosus causes hydatid disease, a major zoonosis. A fatty-acid-binding protein, EgFABP1, is important for the parasite, as it must acquire almost all its lipids from its environment or the host. The structure of EgFABP1 has been solved and refined to 1.6 Å resolution. READ MORE