Search for dissertations about: "substrate-assisted catalysis"

Found 3 swedish dissertations containing the words substrate-assisted catalysis.

2. 1. Rational redesign of Candida antarctica lipase B

   Author : Anders Magnusson; Karl Hult; Bernhard Hauer; KTH; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Biochemistry; Candida antarctica lipase B; rational redesign; secondary alcohols; substrate-assisted catalysis; S-selective; entropy; aldolase; stereospecificity pocket; oxyanion hole.; Biokemi; Biochemistry; Biokemi;

   Abstract : This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. READ MORE

4. 2. Serine Hydrolase Selectivity : Kinetics and applications in organic and analytical chemistry

   Author : Anders Hamberg; Karl Hult; Dick Janssen; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Candida antarctica lipase B; monoacylation of diols; kinetic resolution; thermodynamics in enzyme catalysis; enzyme engineering; Biochemistry; Biokemi;

   Abstract : The substrate selectivities for different serine hydrolases were utilized in various applications, presented in papers I-VI. The articles are discussed in the thesis in view of the kinetics of the enzyme catalysis involved. READ MORE

5. 3. On electrostatic effects, minimal motion and other catalytic strategies used by enzymes

   Author : Per-Olof Syrén; Karl Hult; Jiri Damborsky; KTH; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Biochemistry; Biokemi; SRA - Molecular Bioscience; SRA - Molekylär biovetenskap;

   Abstract : Enzymes are powerful biocatalysts that provide rate accelerations of up to 1019 fold compared to the corresponding uncatalyzed reaction in solution. The origin of the remarkable performance displayed by enzymes has fascinated and puzzled researchers for over a hundred years. READ MORE