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Showing result 1 - 5 of 136 swedish dissertations matching the above criteria.

  2. 1. Towards specific quantification of exosomes using surface-based sensing

    Author : Deborah Rupert; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; label-free; surface plasmon resonance.; exosome; binding rate; antibody; concentration; nanoparticle tracking analysis; quantification; liposome; cluster of differentiation 63 CD63 ; diffusion; specific detection; mass transport limited conditions; extracellular vesicles; surface-based biosensor;

    Abstract : When exosomes were first observed in 1967, they were believed to be cell debris until extensive studies demonstrated key biological functionalities related to their composition and formation mechanism. Exosomes are nanometer-sized lipid vesicles of endocytic origin, with a membrane consisting of lipids and proteins and encapsulating a cargo composed of soluble proteins and genetic material. READ MORE

  4. 2. Physical characterization of exosomes and other extracellular vesicles using surface-based sensing

    Author : Deborah Rupert; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; nanoparticle tracking analysis; exosomes; scattering; surface-based biosensing; optical density; cluster of differentiation 63 CD63 ; liposomes; label-free; fluorescence; surface plasmon resonance; Extracellular vesicles; specificity; antibody; lipid content; single particle; quantification; size distribution; optical waveguide microscopy;

    Abstract : Extracellular vesicles (EVs) are hundred of nanometer sized particles secreted by almost all cell types. Exosomes are one type of EVs today intensively investigated due to the increased evidence suggesting that they play important roles as communicating vehicles in a broad range of physiological processes, pathological conditions and disease diagnostics. READ MORE

  5. 3. Microfluidics in Surface Modified PDMS : Towards Miniaturized Diagnostic Tools

    Author : Sara Thorslund; Fredrik Nikolajeff; Jonas Bergquist; Elisabeth Verpoorte; Uppsala universitet; []
    Keywords : Materials science; µTAS; micro total analysis system; PDMS; poly dimethylsiloxane ; microfluidics; heparin; blood filtration; on-chip; ESI-MS; desalting; QCM-D; biocompatible; CD4; capillary flow; lab-on-chip; microfabrication; enrichment; point-of-care; hydrophilic; oxidation; Materialvetenskap;

    Abstract : There is a strong trend in fabricating miniaturized total analytical systems, µTAS, for various biochemical and cell biology applications. These miniaturized systems could e.g. gain better separation performances, be faster, consume less expensive reagents and be used for studies that are difficult to access in the macro world. READ MORE

  6. 4. Cardiomyocyte Cell Cycle, Renewal and Isolation

    Author : Stuart Walsh; Stamcellscentrum (SCC); []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; binucleation; Cardiomyocytes; surface marker; troponin-T; mitosis; microarray; cell cycle; FACS;

    Abstract : Heart disease results from the loss of cardiomyocytes following myocardial damage and is a leading cause of death worldwide. Stimulating the endogenous regeneration of cardiomyocytes or isolating them from in vitro culture is an attractive therapeutic strategy. READ MORE

  7. 5. On osseointegration in response to controlled surface nanotopography

    Author : Dimitrios Karazisis; Göteborgs universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; in vivo; nanotopography; osseointegration; topography;

    Abstract : Knowledge about the biological responses provoked by the surface modification of titanium implants on the nanoscale is still in its infancy. Although in vitro studies claim superior effects considering higher adhesion and proliferation of osteoblasts in the short term and even differentiation towards the osteogenic cell lineage in the long term, these responses do not necessarily reflect the actual outcome in the complex in vivo environment. READ MORE