Search for dissertations about: "thermus"
Showing result 1 - 5 of 13 swedish dissertations containing the word thermus.
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1. Phylogenetic characterization of microbial mats and isolation of Thermus spp. and sulfur-oxidizing bacteria from Icelandic hot springs
Abstract : Until recently, the only way to describe microbial communities was by cultivation. This is a major obstacle because typically only a small fraction of the microbes can be cultivated by standard techniques. However, the use of rRNA and molecular phylogenetic techniques has allowed us to bypass this limitation. READ MORE
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2. Diversity of thermostable DNA enzymes from Icelandic hot springs
Abstract : The diversities of bacteria as well as thermostable DNA enzymes in Icelandic hot springs were studied using different techniques. First of all the bacterial diversity of different microbial mats as well as sediment samples was described by using both molecular methods and cultivation methods. READ MORE
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3. Protein-RNA interplay in translation. Structural studies of RRF, SelB and L1
Abstract : Translation is a central biological process, that is catalysed by a machinery consisting of RNA and protein. In this thesis three different protein components of the bacterial protein synthesis system have been structurally studied. READ MORE
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4. Protein production, characterization and structure determination in structural genomics
Abstract : This thesis covers the process from expression of a heterologous gene in Escherichia coli to structure determination of a protein by nuclear magnetic resonance (NMR) spectroscopy. The first part concerns structural genomics-related parallel screening studies on the effect of fusion tags (in particular the His tag) on protein solubility and the use of fusion tags in fast, parallel purification protocols intended for initial biophysical characterization of human proteins produced in E. READ MORE
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5. Proton transfer in nitric oxide reducing heme-copper oxidases
Abstract : Heme-copper oxidases (HCuOs) are best known as terminal oxidases in the aerobic respiratory chain, in which they catalyze the reduction of oxygen to water. By receiving protons and electrons from opposite sides of the membrane as well as pumping protons, HCuOs contribute to the electrochemical proton gradient over the membrane that can be used for ATP synthesis. READ MORE