Search for dissertations about: "thesis fluorescent dyes"

Showing result 1 - 5 of 40 swedish dissertations containing the words thesis fluorescent dyes.

  2. 1. Groove-binding Unsymmetrical Cyanine Dyes for DNA Applications

    Author : Maja Eriksson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; polarized spectroscopy; bacteriophage T5; cryo-transmission electron microscopy; dynamic light scattering; DNA; intercalation; cyanine dyes; groove-binding; fluorescence spectroscopy; gel electrophoresis;

    Abstract : This work focuses on interactions between DNA and a group of newly developed unsymmetrical cyanine dyes. The aim of the work was to discover fluorescent dyes that can be used in biophysical studies of natural DNA where minimum perturbation of the DNA-helix is essential, where a slow dissociation rate of the dyes is needed and where the ionic strength is an important parameter. READ MORE

  4. 2. Tailoring Fluorescent Probes for Organelle-Specific Imaging and Sensing

    Author : Xin Zhang; Zhangjun Hu; Kajsa Uvdal; Fredrik Almquist; Linköpings universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Fluorescent imaging; Ratiometric Nanoprobe; Autophagy; Apoptosis; FRET; Fluorescerande avbildning; Ratiometric Nanoprobe; Autophagy; Apoptosis; FRET;

    Abstract : Exploring and visualizing biological and pathological processes in living cells are useful for both fundamental research and applications. An understanding on the molecular level for these processes with focus on tracking biomarkers, collecting information about their surroundings, and uncovering essential molecular pathways and functions in live cells are of vital interest for cell biological study and clinical diagnosis application. READ MORE

  5. 3. Expanding the alphabet of fluorescent nucleic acid base analogues: Characterization of new members

    Author : Anke Dierckx; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescent adenine analogue; fluorescent DNA base analogue; duplex stability; Fluorescence quantum yield; circular dichroism; B-DNA;

    Abstract : Fluorescence has proven to be a powerful tool for studying structure, dynamics and interactions of biomolecules such as proteins, RNA and DNA. However, since the natural DNA/RNA nucleobases are virtually non-fluorescent, there is a need for developing fluorescent nucleic acid markers. READ MORE

  6. 4. Characterization of Fluorescent Dyes for Biochemical Applications. Chemometric Analysis of Spectroscopic Data

    Author : Jan Nygren; Chalmers tekniska högskola; []
    Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; principal component analysis; fluorescein; chemometrics; optical spectroscopy; fluorophores; thiazole orange;

    Abstract : Fluorescent dyes are used in a number of different biochemical applications to visualize and probe biomolecules. The aim of this work was to characterize the spectroscopic properties of two such dyes, fluorescein and thiazole orange, and to develop chemometric methods to extract further information from these data. READ MORE

  7. 5. Pitfalls in β-cell ion imaging with fluorescent indicators and their use for real-time detection of somatostatin secretion

    Author : Mingyu Yang; Anders Tengholm; Enming Zhang; Uppsala universitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; β-cells; cytoplasmic Ca²⁺; cytoplasmic pH; fluorescent indicators; pancreatic islets; R-GECO1; somatostatin; type 2 diabetes; Molekylär cellbiologi; Molecular Cellbiology;

    Abstract : Fluorescent ion indicators have become indispensable tools in cell physiology. Dye indicators can easily be loaded into most types of cells, while genetically encoded indicators are advantageous in allowing specific cellular or subcellular targeting. READ MORE