Search for dissertations about: "thesis method development on hplc"

Showing result 1 - 5 of 30 swedish dissertations containing the words thesis method development on hplc.

  1. 1. Development and improvement of methods for characterization of HPLC stationary phases

    University dissertation from Uppsala universitet

    Author : Torgny Undin; Uppsala universitet.; [2011]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; Adsorption isotherms; Elution by characteristic points; Inflection points; Single component adsorption; Elution by characteristic points method; Slope data; ECP-slope method; Adsorption; Adsorption energy distribution; Adsorption isotherms; AED; Characterization of adsorption processes; Chromatographic analysis; Chromatography; Hydrophobic-subtraction method HSM ; Hydrophobicity; Linear methods; Linear Models; Liquid chromatography; metoprolol; Non-linear methods; Nonlinear methods; phenol; priority journal; propranolol; Retention mechanism; reversed phase liquid chromatography; Solvation; Subtraction method; Thermodynamics; Chemistry with specialization in Surface Biotechnology; Kemi med inriktning mot ytbioteknik;

    Abstract : High Performance Liquid Chromatography (HPLC) is a widely used tech-nique both for detecting and purifying substances in academy and in the industry. In order to facilitate the use of, and knowledge in HPLC, character-ization of stationary phases is of utmost importance. READ MORE

  2. 2. Mass Spectrometry of Biologically Active Small Molecules Focusing on polyphenols, alkaloids and amino acids

    University dissertation from Stockholm : Department of Analytic Chemistry, Stockholm University

    Author : Zdeněk Spáčil; Stockholms universitet.; [2010]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; LC-MS; LDI; Mass spectrometry; HPLC; UHPLC; polyphenols; phenolic acids; BMAA; NATURAL SCIENCES Chemistry Analytical chemistry Separation methods; NATURVETENSKAP Kemi Analytisk kemi Separationsmetoder; NATURAL SCIENCES Chemistry Analytical chemistry; NATURVETENSKAP Kemi Analytisk kemi; Analytical Chemistry; analytisk kemi;

    Abstract : The foci of this dissertation are on advanced liquid chromatography (LC) separation and mass spectrometry (MS) techniques for the analysis of small bioactive molecules. In addition to discussing general aspects of such techniques the results from analyses of polyphenols (PPs), alkaloids and amino acids published in five appended studies are presented and discussed. READ MORE

  3. 3. Development and Validation of HPLC Methods for Analytical and Preparative Purposes

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Johan Lindholm; Uppsala universitet.; [2004]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Chemistry; High performance liquid chromatography HPLC ; Analytical biotechnology; Sample preparation; Diode-array-detection DAD ; Mass spectrometry MS ; Validation; Preparative chromatography; Competitive isotherm parameters; Perturbation peak PP method; Kemi; NATURAL SCIENCES Chemistry; NATURVETENSKAP Kemi;

    Abstract : This thesis concerns the development and validation of high performance liquid chromatography (HPLC) methods aimed for two industrially important areas: (i) analysis of biotechnological synthesis and (ii) determination of adsorption isotherm parameters. There is today a lack of detailed recommendations for analytical procedures in the field of biotechnological production of drugs. READ MORE

  4. 4. Adsorption Isotherm Parameter Estimation in Nonlinear Liquid Chromatography

    University dissertation from Uppsala : Acta Universitatis Upsaliensis

    Author : Patrik Forssén; Uppsala universitet.; Uppsala universitet.; [2005]
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; High performance liquid chromatography HPLC ; Preparative chromatography; Competitive adsorption isotherm parameters; Perturbation peak PP method; Inverse method IM ; Computer simulation; Parameter estimation; Numerical method; Scientific Computing; Beräkningsvetenskap;

    Abstract : This thesis concerns the development and validation of methods for the industrially important area of adsorption isotherm parameter estimation in preparative, nonlinear high performance liquid chromatography (HPLC). Preparative chromatography is a powerful separation method to get pure compounds from more or less complex liquid mixtures, e.g. READ MORE

  5. 5. Method development for analysis of 8-oxodG as a biomarker for oxidative stress

    University dissertation from Stockholm : Karolinska Institutet, Biosciences and Nutrition

    Author : Tim Hofer; Karolinska Institutet.; Karolinska Institutet.; [2001]
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES;

    Abstract : Oxidative damage to DNA gives oxidation of DNA bases and strand breaks that can cause mutations leading to cancer. Various forms of agents are capable of oxidising DNA in vivo including peroxides, singlet oxygen and UV- or gamma-irradiation. Oxidation of DNA bases occurs mainly at dG, giving stable DNA adducts such as 8-oxodG and FapydG. READ MORE