Search for dissertations about: "thesis method development on hplc"

Showing result 11 - 15 of 35 swedish dissertations containing the words thesis method development on hplc.

  2. 11. Progress of Weak Affinity Chromatography as a Tool in Drug Development

    Author : Elinor Meiby; Sten Ohlson; Manfred Raida; Linnéuniversitetet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; weak affinity chromatography; fragment based drug discovery; fragment screening; thrombin; trypsin; cyclin G-associated kinase; Hsp90; Pin1; cyclooxygenase; lipodisks; Biokemi; Biochemistry;

    Abstract : Weak Affinity Chromatography (WAC) is a technology that was developed to analyse weak (KD > 10-5 M) although selective interactions between biomolecules. The focus of this thesis was to develop this method for various applications in the drug development process.Fragment Based Drug Discovery is a new approach in finding new small molecular drugs. READ MORE

  4. 12. High-performance liquid chromatography for analysis of 32P-postlabeled DNA adducts

    Author : Magnus Zeisig; Karolinska Institutet; Karolinska Institutet; []
    Keywords : DNA adducts; 32P-postlabeling; HPLC; method development;

    Abstract : The formation of DNA adducts, i.e. the covalent binding of chemicals and chemical groups to DNA, is believed to be an important step in chemical carciogenesis. DNA adducts are usually formed at very low levels which requires very sensitive methods to analyze them. READ MORE

  5. 13. Determination of Fat-Soluble Vitamins in Foods Based on Supercritical Fluid Extraction

    Author : Charlotta Turner; Kemiska institutionen; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Supercritical fluid extraction; Solvent collection; Solid phase trapping; SFE; Selectivity; Retinol; Milk powder; Lipase; Food; Enzymatic hydrolysis; Alcoholysis; Candida antarctica; Tocopherol; Vitamins; Analytical chemistry; Analytisk kemi;

    Abstract : Sample clean-up methodologies based on supercritical fluid extraction (SFE) have been developed for determination of fat-soluble vitamins in foods. This thesis focuses on method development, stressing important issues concerning sample pre-treatment, extraction conditions and collection parameters. READ MORE

  6. 14. Improved Techniques for Protein Analysis Focusing on Membrane Proteins and Hydrophobic Peptides

    Author : Theres Redeby; Åsa Emmer; Jonas ergquist; KTH; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Integral membrane proteins; Hydrophobic peptides; Sample handling; MALDI-TOF-MS; Structured sample support; CE; fraction collection; microchannel; Analytical chemistry; Analytisk kemi;

    Abstract : In this thesis, the vital cell functions performed by integral membrane proteins (IMPs) are briefly discussed. Such proteins are under-represented in most protein studies due to the hydrophobic nature of IMPs, which seriously complicate their solubilization, sample handling, preparation, separation and analysis. READ MORE

  7. 15. Determination of folate for food composition data : development and improvement of analytical methods

    Author : Hanna Sara Strandler; Sveriges lantbruksuniversitet; Sveriges lantbruksuniversitet; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; LANTBRUKSVETENSKAPER; AGRICULTURAL SCIENCES;

    Abstract : Folate is B vitamin that is required for maintaining normal blood status. Recommendations on daily intake are based on information on blood folate status in relation to folate intake and bioavailability. Folate intake from a diet is evaluated by folate content in the food consumed. READ MORE