Search for dissertations about: "thesis trna modification"

Showing result 1 - 5 of 17 swedish dissertations containing the words thesis trna modification.

  2. 1. Choice of tRNA on Translating Ribosomes

    Author : Elli Bouakaz; Måns Ehrenberg; Christian Spahn; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Ribosome; Translation; Accuracy; Proofreading; tRNA; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Abstract : This thesis addresses different aspects of the question about accuracy of protein synthesis: i) the mechanism of tRNA selection during translation ii) study of ribosomal mutations that affect accuracy and iii) the choice of aminoacyl-tRNA isoacceptors on synonymous codons.By measuring the codon reading efficiencies of cognate and near-cognate ternary complexes we demonstrate that in optimal physiological conditions accuracy of substrate selection is much higher than previously reported; that during translation the ribosomal A site is not blocked by unspecific binding of the non-cognate tRNAs which would inhibit the speed of protein synthesis. READ MORE

  4. 2. Functional aspects of modified nucleosides in tRNA

    Author : Hao Xu; Anders S. Byström; Damien Hermand; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; tRNA modification; frameshifting; Elongator complex; Elp1p; Prb1p; proteolysis; glioma; TRM6 61; PKCα;

    Abstract : Transfer ribonucleic acids (tRNAs) are extensively modified, especially their anticodon loops. Modifications at position 34 (wobble base) and 37 in these loops affect the tRNAs’ decoding ability, while modifications outside the anticodon loops, e.g. m1A58 of tRNAMeti, may be crucial for tRNA structure or stability. READ MORE

  5. 3. Formation of Thiolated Nucleosides in tRNA in Salmonella enterica serovar typhimurium

    Author : Hans Lundgren; Glenn Björk; Charles Lauhon; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; : tRNA; modified nucleosides; thiolation; sulfur metabolism; Salmonella; Molecular biology; Molekylärbiologi;

    Abstract : The presence and synthesis of transfer RNA (tRNA) is highly conserved in all organisms and a lot of genetic material is dedicated to its synthesis. tRNA contains a large number of modified nucleosides and several diverse functions have been found but much about their function is still unknown. READ MORE

  6. 4. Frameshifting as a tool in analysis of transfer RNA modification and translation

    Author : Ramune Leipuviene; Glenn Björk; Diana Downs; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; minor tRNA; frameshifting; suppression; L9; hopping; tRNA thiolation; [Fe-S] cluster; [Fe-S] protein; TtcA; Molekylärbiologi; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : Studies of ribosomal reading frame maintenance are often based on frameshift mutation suppression experiments. In this thesis, suppression of a frameshift mutation in Salmonella enterica serovar Typhimurium by a tRNA and a ribosomal protein are described. READ MORE

  7. 5. Influence of the tRNA modification ms2i6A on codon translation

    Author : Irene M. Díaz Bermúdez; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP;

    Abstract : .... READ MORE