Search for dissertations about: "total internal reflection TIRF microscopy"

Showing result 1 - 5 of 11 swedish dissertations containing the words total internal reflection TIRF microscopy.

2. 1. Caveolae associated proteins and how they effect caveolae dynamics

   Author : Björn Morén; Richard Lundmark; Volker Haucke; Umeå universitet; []
   Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Caveolae; caveolin; EHD2; cavin; microdomain; microscopy; TIRF; AFM; medicinsk biokemi; Medical Biochemistry;

   Abstract : Caveolae are a type of invaginated membrane domain that has been shown to be involved in several disease states, including lipodystrophy, muscular dystrophies and cancer. Several of these diseases are caused by the lack of caveolae or caveolae-related signaling deficiencies in the tissues in which the caveolar domain are abundant such as lung, adipose, muscle and their related endothelial cells. READ MORE

4. 2. DNA-Controlled Lipid-Membrane Fusion

   Author : Lisa Simonsson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; exocytosis; vesicles; DNA; FRET; fluorescence resonance energy transfer; model system; total internal reflection fluorescence microscopy; TIRF microscopy; self-assembly; site-specificity; diffusion; biomembranes; lipid bilayer; membrane fusion;

   Abstract : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. We have demonstrated that short DNA oligonucleotides, membrane-attached via CH in an orientation that mimics the overall zipperlike architecture of fusion-inducing proteins, induce fusion of both suspended vesicles and vesicles site-specifically tethered to SLBs. READ MORE

5. 3. Lipid Vesicle Fusion: Investigation, Generation and Manipulation of Cell-Membrane Mimics

   Author : Lisa Simonsson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; diffusion; spreading lipid bilayers; cell mimic; micromanipulation; lipid bilayer; microfluidics; site-specificity; DNA; membrane-receptor interactions; total internal reflection fluorescence microscopy; model system; self-assembly; TIRF microscopy; FRET; exocytosis; vesicles; fluorescence resonance energy transfer; biomembranes; amperometry; membrane fusion;

   Abstract : Membrane fusion is essential for nerve-cell communication, for protein transport between cell organelles and the cell-membrane and for enabling the merger between virus and host membranes during virus infection. In this work, cell-membrane mimics were constructed and evaluated as models for studies of the membrane-fusion process. READ MORE

6. 4. Characterization of artificial and biological lipid vesicles using TIRF and SPR

   Author : Thomas Olsson; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; membrane permeability; chromaffin vesicle; fluorescence; total internal reflection TIRF microscopy; surface plasmon resonance SPR ; vesicle; osmotic shock; cell membrane; liposome; nanoparticle tracking analysis NTA ; large dense core vesicles LDCVs ;

   Abstract : Synthetic lipid vesicles serve as important mimics of cells and the natural membranes that they are enclosed by. As such they are frequently used as simplified models of the highly complex cell membrane to aid in-depth physicochemical and biological characterization of this essential biological structure. READ MORE

7. 5. Fabrication, development and characterization of a waveguide microscopy device for biological applications

   Author : Mokhtar Mapar; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; NATURVETENSKAP; NATURAL SCIENCES; Image processing; Lipid bilayer; Waveguide scattering microscopy; Waveguide fluorescent microscopy; Virus; Nanofabrication;

   Abstract : Our understanding of biological systems has advanced significantly thanks to the development of microscopy methods and suitable biological assays. Surface based methods have increasingly gained interest thanks to their superior sensitivity and ease of use. READ MORE