Search for dissertations about: "trans-translation"

Found 3 swedish dissertations containing the word trans-translation.

  1. 1. Finding the unknowns in trans-translation

    Author : Natalia Ivanova; Måns Ehrenberg; Nenad Ban; Uppsala universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; trans-translation; tmRNA; SmpB; kinetics; footprinting; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Abstract : Ribosomes stalled on problematic mRNAs can be rescued by a mechanism called trans-translation. This mechanism employs a dual transfer-messenger RNA molecule (tmRNA) together with a helper protein (SmpB). In this work we have used an in vitro translation system with pure components to further clarify the roles of tmRNA and SmpB in trans-translation. READ MORE

  2. 2. Studies on Translation Initiation and trans-Translation

    Author : Serhiy Surkov; Leif Isaksson; Udo Bläsi; Stockholms universitet; []
    Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; E. coli; ribosome; IF1; tmRNA; trans-translaton; Molecular biology; Molekylärbiologi; molekylärgenetik; Molecular Genetics;

    Abstract :  Translation initiation factor 1 (IF1) is an essential bacterial protein, without any clearly defined function in translation initiation. Same point mutants in this protein exhibit cold-sensitivity. In addition, a sequence specific increase in test protein expression has been observed for these mutants. READ MORE

  3. 3. Single-particle cryo-electron microscopy of macromolecular assemblies

    Author : Kimberley Cheng; Hans Hebert; Roland Beckmann; KTH; []

    Abstract : In this thesis, single-particle cryo-electron microscopy (cryo-EM) was used to study the structure of three macromolecular assemblies: the two hemocyanin isoforms from Rapana thomasiana, the Pyrococcus furiosus chaperonin, and the ribosome from Escherichia coli. Hemocyanins are large respiratory proteins in arthropods and molluscs. READ MORE