Search for dissertations about: "triplex DNA"

Showing result 1 - 5 of 17 swedish dissertations containing the words triplex DNA.

2. 1. Development of benzoquinoquinoxaline derivatives as triplex-specific probes : Recognition of DNA structures at repeats sequences

   Author : Helen Bergquist; Rula Zain; Keith Fox; Stockholms universitet; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; Triplex; H-DNA; BQQ; BQQ-OP; BQQ-Bodipy; triplet repeat; DNA; non-B-DNA; pkd1; frataxin; Friedreich s ataxia; Molecular biology; Molekylärbiologi; Molecular Biology; molekylärbiologi;

   Abstract : Repeat sequences are associated with several human diseases, such as Friedreich’s ataxia, polycystic kidney disease and cancer. These sequences can form non-B-DNA structures, including triplex (H-DNA) DNA, and are associated with genomic instability and altered gene expression. READ MORE

4. 2. Structural Adaptation of DNA to Small Ligands and Recombination Proteins

   Author : Hye-Kyung Kim; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; triplex DNA; groove binders; intercalators; SANS; DNA; optical spectroscopy; recombination proteins;

   Abstract : In order to contribute to the understanding of mechanisms of recognition in nucleic acid systems, the interaction of DNA with a variety of ligands, representing different cases of adaptation of the double-helix structure, was studied using flow linear dichroism (LD), circular dichroism (CD), fluorescence and absorption spectroscopic techniques. A chiral hydrophobic metal complex, [Ru(dpphen)3]2+ was examined, since this had been reported to be the most efficient molecule to discriminate DNA handedness. READ MORE

5. 3. Fixation of Functionalized DNA Nanostructures

   Author : Erik Lundberg; Chalmers tekniska högskola; []
   Keywords : TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; triplex recognition; dna; self-assembly; gel electrophoresis; nanotechnology; cross-linking; spectroscopy; fixation; fret; click chemistry;

   Abstract : This thesis features results from two different studies on a fully addressable non-repetitive network on the nanometre scale, assembled by synthetic DNA molecules. A bio-inspired molecular approach, based on principles of self-assembly, has brought about a paradigm shift in nanoscience, from a top-down to a bottom-up perspective. READ MORE

6. 4. Bottom-up Fabrication of Functional DNA Nanostructures

   Author : Erik Lundberg; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; click chemistry; gel electrophoresis; self-assembly; cross-linking; lipid membrane; fixation; linear dichroism; nanotechnology; spectroscopy; fret; dna; triplex recognition;

   Abstract : This thesis demonstrates bottom-up fabrication of a fully addressable non-repetitive network on the nanometer scale, assembled by synthetic DNA molecules. Each side constitutes a unique sequence of 10 bases, i.e. 3. READ MORE

7. 5. Intelligent Nucleic Acid Interactions with Peptide Nucleic Acid (PNA) and Recombination Enzyme RecA

   Author : Pernilla Wittung; Chalmers tekniska högskola; []
   Keywords : NATURVETENSKAP; NATURAL SCIENCES; fluorescence; RecA; homologous recombination; circular dichroism; DNA analogs; -anti-BPDE; spectroscopy; PNA; DNA-protein interactions;

   Abstract : The work presented in this thesis focuses on interactions between nucleic-acids in biological systems, particularly interactions of the promising drug-candidate peptide nucleic acid (PNA), and on protein-DNA intermediates during recombination. Several experimental techniques, mainly circular dichroism and fluorescence spectroscopy, have been used to characterize the interactions of interest. READ MORE