Search for dissertations about: "whole cell patch clamp."
Showing result 1 - 5 of 30 swedish dissertations containing the words whole cell patch clamp..
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1. In vivo properties of neural stem cells after transplantation into the rat brain-Studies of phenotypic differentiation and functional integration using cell-specific labelling and electrophysiological techniques
Abstract : In the present thesis, we have examined the in vivo properties of in vitro expanded human and rat neural stem-and progenitor cells after transplantation into the neonatal and adult rat brain. The survival and differentiation of the grafted cells were assessed using species-specific antisera, and pre-labelling with the reporter gene green fluorescent protein (GFP). READ MORE
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2. Electrophysiology of pancreatic A-, B- and D-cells studied in intact mouse islets of Langerhans
Abstract : Abstract The electrophysiological properties of insulin-secreting b-cells, glucagon-releasing a-cells and somatostation-producing d-cells in intact mouse pancreatic islets were investigated using the perforated patch whole-cell technique. It is demonstrated that it is possible to functionally identify the different cell types in situ. READ MORE
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3. Synaptic Integration of Hippocampal Neurons Generated in the Adult Brain: Influence of a Pathological Environment
Abstract : The adult brain constantly produces new neurons from endogenous neural progenitor cells located in at least two regions, the subgranular zone (SGZ) in dentate gyrus of the hippocampus, and the subventricular zone (SVZ). Major efforts are underway to discover the functional significance of new neurons in the adult brain. READ MORE
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4. Effects of Ca2+, microRNAs, and rosuvastatin on insulin-secreting beta cell function
Abstract : Type 2 diabetes (T2D) is a condition of high blood glucose levels due to insulin resistance and defective insulin secretion. Impaired insulin secretion plays a major role in the pathophysiology of T2D, it is mainly attributed to beta cell function i.e. failure to secrete insulin or reduced beta cell mass. READ MORE
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5. Short- and long-term effects of long-chain free fatty acids on pancreatic α- and β-cell function
Abstract : We have investigated the in vitro effects of long-chain free fatty acids (FFAs) on mouse pancreatic α- and β-cells by a combination of electrophysiological recordings, intracellular Ca2+ ([Ca2+]i) microfluorimetry and hormone release measurements. Acute addition of the saturated FFA palmitate to intact islets doubled insulin as well as glucagon secretion in the presence of 1 or 15 mM glucose and the FFA remained capable of enhancing release of both hormones in islets depolarised with high K+ in the presence of the KATP channel opener diazoxide, indicating that alterations of ATP sensitive potassium (KATP) channel activity is not part of the effect. READ MORE