Search for dissertations about: "wobble base pair"

Found 3 swedish dissertations containing the words wobble base pair.

  2. 1. Nucleic Acids as Drug Targets - Influence of Platinum Complexes on Duplex Stability and Binding Kinetics

    Author : Christopher Polonyi; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Anticancer drugs; platinum complexes; cisplatin; oxaliplatin; nucleic acid; RNA; DNA; duplex melting; thermodynamic stability; kinetics; non-coding RNA; miRNA; siRNA; wobble base pair; loop; BRCA1; mimic; luciferase assay;

    Abstract : Gaining a detailed understanding of how human cells are affected by metal-based drugs is crucial, since these drugs have shown great potential in cancer treatment. Cisplatin is used to treat many different types of cancer, e.g. testicular, ovarian, melanoma, bladder, non-small cell lung cancer, small cell lung cancer, myelomas and lymphomas. READ MORE

  4. 2. Function of wobble nucleoside modifications in tRNAs of Salmonella enterica Serovar Typhimurium

    Author : Peng Chen; Glenn R. Björk; Paul Agris; Umeå universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; tRNA; wobble nucleoside; frameshifting; translation; Molekylärbiologi; Molecular biology; Molekylärbiologi; molekylärbiologi; Molecular Biology;

    Abstract : Transfer RNA from all organisms has modified nucleosides and position 34 (the wobble position) is one of the most extensively modified positions. Some wobble nucleoside modifications restrict codon choice (e.g. 5-methylaminomethyl-2-thiouridine, mnm5s2U) while some extend the decoding capacity (e. READ MORE

  5. 3. Choice of tRNA on Translating Ribosomes

    Author : Elli Bouakaz; Måns Ehrenberg; Christian Spahn; Uppsala universitet; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Molecular biology; Ribosome; Translation; Accuracy; Proofreading; tRNA; Molekylärbiologi; Molecular biology; Molekylärbiologi;

    Abstract : This thesis addresses different aspects of the question about accuracy of protein synthesis: i) the mechanism of tRNA selection during translation ii) study of ribosomal mutations that affect accuracy and iii) the choice of aminoacyl-tRNA isoacceptors on synonymous codons.By measuring the codon reading efficiencies of cognate and near-cognate ternary complexes we demonstrate that in optimal physiological conditions accuracy of substrate selection is much higher than previously reported; that during translation the ribosomal A site is not blocked by unspecific binding of the non-cognate tRNAs which would inhibit the speed of protein synthesis. READ MORE