Cellular Uptake of Cystatin C. Subcellular localisation and intracellular effects of a secreted cysteine protease inhibitor

University dissertation from Division of Clinical Chemistry and Pharmacology, Faculty of Medicine, Lund University

Abstract: Popular Abstract in English The secreted cysteine protease inhibitor cystatin C has been thought to exert its functions extracellularly, as a defense against enzymes from leaking lysosomes or invading pathogens. In this thesis we demonstrate that regulation of intracellular proteases by cystatin C is possible, as a result of cellular uptake, and that the uptake can be modulated by molecular engineering of wild-type cystatin C. The uptake was seen in different epithelial and neuroblastoma cancer cell lines and was shown by various techniques, including flow cytometry, confocal microscopy, ELISA and Western blotting. Intracellular cysteine proteases involved in cancer-promoting processes may thus be controlled by cystatin C uptake.

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