Sampling and Selective Detection of Peptides and Proteins Emphazing Microdialysis,Bio-recognition Assays and MALDI-TOF MS

University dissertation from Analytical Chemistry (S/LTH)

Author: Sven Kjellström; Lunds Universitet.; Lund University.; [2000]

Keywords: NATURVETENSKAP; NATURAL SCIENCES; protein identification cyclodextrines m-dispenser push-pull microdialysis microdialysis sampling MALDI-TOF MS Interleukin-8 Flow immuno assays Interleukin-10 Analytical chemistry Analytisk kemi;

Abstract: The use of microdialysis sampling and flow immunochemical detection as well as MALDI-TOF MS for peptide and protein analysis is described. Sampling of peptides and smaller proteins was investigated with different membranes. Cyclodextrines additives and an on-line push-pull microdialysis sampling method was used with the result of a higher sampling efficiency compared to conventional microdialysis sampling. Real-time flow immuno assays systems were developed for the quantification of Interleukin-8 and Interleukin10 in cell samples. Optimisation with regards to affinity support selection as well as carrier buffer composition were performed. Connection of capillary LC with MALDI-TOF MS was made utilising an m-dispenser technique enabling sample handling of picoliter volumes. A novel sample preparation technique for the uniform coating of MALDI targets was developed.

  This dissertation MIGHT be available in PDF-format. Check this page to see if it is available for download.