Search for dissertations about: "enzyme staining"

Showing result 1 - 5 of 48 swedish dissertations containing the words enzyme staining.

  2. 1. Optimizing Enzyme Immobilization in Mesoporous Silica - A Spectroscopic Study of the Dynamics and Spatial Distribution of the Confined Enzymes

    Author : Pegah Sadat Nabavi Zadeh; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; NATURVETENSKAP; NATURAL SCIENCES; TEKNIK OCH TEKNOLOGIER; ENGINEERING AND TECHNOLOGY; fluorescence spectroscopy; dynamics; mesoporous silica; Immunogold staining; Transmission electron microscopy; anisotropy; microviscosity; real-time monitoring; Cy5.; Förster resonance energy transfer; Enzyme immobilization; Cy3;

    Abstract : Enzymes as biological catalysts are immobilized in porous materials to improve the enzyme activity and simplify their purification from the reaction media in biocatalytic applications. Mesoporous silica (MPS) particles are used as a promising support material for the immobilization of enzymes. READ MORE

  4. 2. Detection of Biomolecules: Syntheses and Studies of Minor Groove Binding Unsymmetrical Cyanine Dyes and Chromogenic Enzyme Substrates

    Author : Jonas Karlsson; Chalmers tekniska högskola; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; palladium-catalysed cross-couplings; optical spectroscopy; minor groove binding; DNA; cyanine dye; enzyme; intercalation; fluorescence; chromogenic;

    Abstract : Visualisation or detection of biomolecules is usually achieved by the use of fluorescent or highly coloured chromophores. The work presented in this thesis deals with the development of new chromogenic substrates for the detection of enzyme activity, and new DNA-binding fluorescent dyes for staining of DNA. READ MORE

  5. 3. Magnesium Chelatase - a Key Enzyme in Chlorophyll Biosynthesis

    Author : Eva Axelsson; Biokemi och Strukturbiologi; []
    Keywords : NATURVETENSKAP; NATURAL SCIENCES; Kemi; Chemistry; AAA-proteins; Chlorophyll biosynthesis; Magnesium chelatase;

    Abstract : The biosynthesis of chlorophylls is an important process in nature. The green pigments play a fundamental role in absorbing and transferring solar energy to the photosynthetic apparatus. READ MORE

  6. 4. Type 2 Cystatins. Studies on the role of the major cystatin, cystatin C, in mice and on properties and distribution of the novel human cystatins F, G, H and I

    Author : Carl-Michael Nathanson; Avdelningen för klinisk kemi och farmakologi; []
    Keywords : MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; MEDICIN OCH HÄLSOVETENSKAP; MEDICAL AND HEALTH SCIENCES; Clinical chemistry; Klinisk kemi; enzyme kinetics; eosinophilic granulocytes; expression pattern; pleural effusion; knockout mouse; Cysteine peptidase; cystatin;

    Abstract : The aim the thesis work was to study the human cystatins C, E/M, F, G, H and I both in vivo and in vitro. A mouse devoid of cystatin C was used for the cystatin C studies. The levels of cystatins C, E/M and F were measured in pleural effusion samples of patients with various lung disorders. READ MORE

  7. 5. Clusterin in brain and cerebrospinal fluidin Alzheimer s disease

    Author : Anna-Maria Nilselid; Göteborgs universitet; []
    Keywords : Alzheimer s disease; clusterin; apolipoprotein J; SP-40; brain tissue; cerebrospinal fluid; glycosylation; biomarker; enzyme-linked immunosorbent assay;

    Abstract : The apolipoprotein clusterin is suggested to be involved in the pathogenesis of Alzheimer¡¦s disease (AD). At the start of these studies, results had indicated that clusterin was elevated in AD brain, compared with controls. READ MORE